Detailed information of TA system TA038710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2361749..2362374
• Replicon: chromosome
• Accession: NZ_AP024560
• Organism: Escherichia coli strain NIPH17_0020

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FLY91_RS11335
• Protein ID: WP_000911330.1
• Coordinates: 2361976..2362374 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FLY91_RS11330
• Protein ID: WP_000450524.1
• Coordinates: 2361749..2361976 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FLY91_RS11305 (2357552)	2357552..2358022	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FLY91_RS11310 (2358022)	2358022..2358594	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FLY91_RS11315 (2358740)	2358740..2359618	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FLY91_RS11320 (2359635)	2359635..2360669	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FLY91_RS11325 (2360882)	2360882..2361595	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FLY91_RS11330 (2361749)	2361749..2361976	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FLY91_RS11335 (2361976)	2361976..2362374	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
FLY91_RS11340 (2362521)	2362521..2363384	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
FLY91_RS11345 (2363399)	2363399..2365414	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
FLY91_RS11350 (2365488)	2365488..2365829	+	342	Protein_2233	esterase	-
FLY91_RS11355 (2365911)	2365911..2366653	-	743	Protein_2234	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2367275..2368207	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T38710 WP_000911330.1 NZ_AP024560:2361976-2362374 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T38710 NZ_AP024560:2361976-2362374 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT38710 WP_000450524.1 NZ_AP024560:2361749-2361976 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT38710 NZ_AP024560:2361749-2361976 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33