Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1894651..1895305 Replicon chromosome
Accession NZ_AP024560
Organism Escherichia coli strain NIPH17_0020

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FLY91_RS09120 Protein ID WP_000244777.1
Coordinates 1894898..1895305 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FLY91_RS09115 Protein ID WP_000354046.1
Coordinates 1894651..1894917 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLY91_RS09090 (1889939) 1889939..1890682 + 744 Protein_1793 SDR family oxidoreductase -
FLY91_RS09095 (1890739) 1890739..1892172 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
FLY91_RS09100 (1892217) 1892217..1892528 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
FLY91_RS09105 (1892692) 1892692..1893351 + 660 WP_000250274.1 hemolysin III family protein -
FLY91_RS09110 (1893428) 1893428..1894408 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FLY91_RS09115 (1894651) 1894651..1894917 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FLY91_RS09120 (1894898) 1894898..1895305 + 408 WP_000244777.1 protein YgfX Toxin
FLY91_RS09125 (1895345) 1895345..1895866 - 522 WP_001055874.1 flavodoxin FldB -
FLY91_RS09130 (1895978) 1895978..1896828 + 851 Protein_1801 site-specific tyrosine recombinase XerD -
FLY91_RS09135 (1896853) 1896853..1897563 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FLY91_RS09140 (1897569) 1897569..1899302 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T38707 WP_000244777.1 NZ_AP024560:1894898-1895305 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T38707 NZ_AP024560:1894898-1895305 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT38707 WP_000354046.1 NZ_AP024560:1894651-1894917 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT38707 NZ_AP024560:1894651-1894917 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References