Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1332594..1333394 Replicon chromosome
Accession NZ_AP024560
Organism Escherichia coli strain NIPH17_0020

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag FLY91_RS06280 Protein ID WP_000342449.1
Coordinates 1332867..1333394 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag FLY91_RS06275 Protein ID WP_001277108.1
Coordinates 1332594..1332860 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLY91_RS06255 (1328251) 1328251..1328919 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
FLY91_RS06260 (1328912) 1328912..1329970 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
FLY91_RS06265 (1330215) 1330215..1331069 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
FLY91_RS06270 (1331341) 1331341..1332444 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
FLY91_RS06275 (1332594) 1332594..1332860 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
FLY91_RS06280 (1332867) 1332867..1333394 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
FLY91_RS06285 (1333391) 1333391..1333774 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
FLY91_RS06290 (1334198) 1334198..1335307 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
FLY91_RS06295 (1335355) 1335355..1336281 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
FLY91_RS06300 (1336278) 1336278..1337555 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
FLY91_RS06305 (1337552) 1337552..1338319 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T38704 WP_000342449.1 NZ_AP024560:1332867-1333394 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T38704 NZ_AP024560:1332867-1333394 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT38704 WP_001277108.1 NZ_AP024560:1332594-1332860 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT38704 NZ_AP024560:1332594-1332860 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References