Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1311303..1311889 Replicon chromosome
Accession NZ_AP024560
Organism Escherichia coli strain NIPH17_0020

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag FLY91_RS06155 Protein ID WP_042014162.1
Coordinates 1311303..1311533 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag FLY91_RS06160 Protein ID WP_000593555.1
Coordinates 1311530..1311889 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLY91_RS06145 (1307340) 1307340..1310075 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
FLY91_RS06150 (1310075) 1310075..1311199 + 1125 WP_001314210.1 ABC transporter permease -
FLY91_RS06155 (1311303) 1311303..1311533 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
FLY91_RS06160 (1311530) 1311530..1311889 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FLY91_RS06165 (1312009) 1312009..1312410 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
FLY91_RS06170 (1312416) 1312416..1313222 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
FLY91_RS06175 (1313219) 1313219..1313983 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
FLY91_RS06180 (1313983) 1313983..1314816 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
FLY91_RS06185 (1314813) 1314813..1315757 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T38703 WP_042014162.1 NZ_AP024560:1311303-1311533 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T38703 NZ_AP024560:1311303-1311533 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT38703 WP_000593555.1 NZ_AP024560:1311530-1311889 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT38703 NZ_AP024560:1311530-1311889 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References