Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 2766..3532 Replicon chromosome
Accession NZ_AP024556
Organism Vibrio cholerae strain IDH-03506

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag K6J79_RS13810 Protein ID WP_000982260.1
Coordinates 2766..3263 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag K6J79_RS13815 Protein ID WP_000246253.1
Coordinates 3260..3532 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J79_RS13785 384..770 + 387 WP_001015867.1 NUDIX hydrolase -
K6J79_RS13790 827..940 + 114 WP_001900214.1 hypothetical protein -
K6J79_RS13795 949..1170 + 222 WP_032467793.1 DUF1289 domain-containing protein -
K6J79_RS13800 1428..1964 + 537 WP_000469482.1 GNAT family protein -
K6J79_RS13805 2155..2616 + 462 WP_001903091.1 lipocalin family protein -
K6J79_RS18860 2681..2749 + 69 Protein_6 acetyltransferase -
K6J79_RS13810 2766..3263 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
K6J79_RS13815 3260..3532 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
K6J79_RS13820 3925..4050 + 126 WP_001900195.1 DUF645 family protein -
K6J79_RS13830 4300..4746 + 447 WP_000006157.1 hypothetical protein -
K6J79_RS13835 4894..5163 - 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin -
K6J79_RS13840 5156..5434 - 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
K6J79_RS18865 5485..5602 + 118 Protein_13 DUF3265 domain-containing protein -
K6J79_RS13845 5720..5998 + 279 WP_001921601.1 DUF3709 domain-containing protein -
K6J79_RS18870 6358..6456 + 99 WP_223225486.1 DUF3709 domain-containing protein -
K6J79_RS13855 6656..6757 + 102 WP_001921603.1 hypothetical protein -
K6J79_RS13860 6747..7133 + 387 WP_000703163.1 VOC family protein -
K6J79_RS13865 7328..7579 + 252 WP_001890111.1 TIGR03643 family protein -
K6J79_RS18875 7552..7701 + 150 WP_071908333.1 DUF3265 domain-containing protein -
K6J79_RS13870 7793..8035 + 243 WP_000107461.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - cqsA / ugd / htpB / hlyA / hcp-2 / vgrG-2 / vipA/mglA / vipB/mglB / VCA0109 / vasA / vasB / vasC / vasD / vasE / vasF / clpB/vasG / vasH / vasI / vasJ / icmF/vasK / vasL / vgrG-3 / hlyA 3..1061958 1061955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T38687 WP_000982260.1 NZ_AP024556:c3263-2766 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T38687 NZ_AP024556:c3263-2766 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT38687 WP_000246253.1 NZ_AP024556:c3532-3260 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT38687 NZ_AP024556:c3532-3260 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References