Detailed information of TA system TA038657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 709223..709751
• Replicon: chromosome
• Accession: NZ_AP024552
• Organism: Vibrio cholerae strain IDH-02387

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: K6J81_RS17395
• Protein ID: WP_000221352.1
• Coordinates: 709461..709751 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: K6J81_RS17390
• Protein ID: WP_000213183.1
• Coordinates: 709223..709471 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J81_RS17360	705030..705224	-	195	Protein_638	hypothetical protein	-
K6J81_RS17365	705380..705880	-	501	WP_000789723.1	hypothetical protein	-
K6J81_RS17370	706018..706548	-	531	WP_000415563.1	hypothetical protein	-
K6J81_RS17375	706699..707094	-	396	WP_000649527.1	DUF3465 domain-containing protein	-
K6J81_RS17380	707302..707727	-	426	WP_000415752.1	hypothetical protein	-
K6J81_RS17385	707999..708652	-	654	WP_000036957.1	site-specific DNA-methyltransferase	-
K6J81_RS17390	709223..709471	+	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K6J81_RS17395	709461..709751	+	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J81_RS17400	709887..710348	-	462	WP_001911752.1	lipocalin family protein	-
K6J81_RS17405	710539..711015	-	477	WP_001047180.1	GNAT family N-acetyltransferase	-
K6J81_RS19275	711043..711153	-	111	WP_071917832.1	DUF3265 domain-containing protein	-
K6J81_RS17410	711150..712079	-	930	WP_000335802.1	hypothetical protein	-
K6J81_RS17415	712199..712645	-	447	WP_000128977.1	hypothetical protein	-
K6J81_RS17420	712798..713802	-	1005	WP_000964925.1	LD-carboxypeptidase	-
K6J81_RS19280	713990..714106	-	117	WP_001889170.1	hypothetical protein	-
K6J81_RS17430	714069..714194	-	126	WP_001944767.1	DUF645 family protein	-
K6J81_RS19285	714222..714332	-	111	Protein_654	acetyltransferase	-
K6J81_RS19290	714371..714481	-	111	WP_134814058.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	672778..725906	53128
	-	flank	IS/Tn	-	-	703399..705009	1610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T38657 WP_000221352.1 NZ_AP024552:709461-709751 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T38657 NZ_AP024552:709461-709751 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT38657 WP_000213183.1 NZ_AP024552:709223-709471 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT38657 NZ_AP024552:709223-709471 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8