Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 84941..85542 Replicon plasmid pE189-1
Accession NZ_AP024522
Organism Escherichia coli strain E189

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag LDO00_RS24665 Protein ID WP_001216034.1
Coordinates 84941..85321 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LDO00_RS24670 Protein ID WP_001190712.1
Coordinates 85321..85542 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO00_RS24635 80041..80160 - 120 WP_071527722.1 ash family protein -
LDO00_RS24640 (NIAHE189_47220) 80382..81866 - 1485 WP_000124159.1 hypothetical protein -
LDO00_RS24645 (NIAHE189_47230) 81866..83059 - 1194 WP_166465177.1 terminase -
LDO00_RS24650 (NIAHE189_47240) 83145..83597 - 453 WP_001326849.1 late promoter-activating protein -
LDO00_RS24655 (NIAHE189_47250) 83686..84729 - 1044 WP_001561086.1 DUF968 domain-containing protein -
LDO00_RS24660 (NIAHE189_47260) 84757..84936 - 180 WP_000113018.1 hypothetical protein -
LDO00_RS24665 (NIAHE189_47270) 84941..85321 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LDO00_RS24670 (NIAHE189_47280) 85321..85542 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LDO00_RS24675 (NIAHE189_47290) 85725..87281 + 1557 WP_166465176.1 type I restriction-modification system subunit M -
LDO00_RS24680 (NIAHE189_47300) 87278..88480 + 1203 WP_166465175.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..98724 98724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T38607 WP_001216034.1 NZ_AP024522:c85321-84941 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T38607 NZ_AP024522:c85321-84941 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT38607 WP_001190712.1 NZ_AP024522:c85542-85321 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT38607 NZ_AP024522:c85542-85321 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References