Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4918319..4918895 Replicon chromosome
Accession NZ_AP024521
Organism Escherichia coli strain E189

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag LDO00_RS23920 Protein ID WP_001350372.1
Coordinates 4918319..4918606 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag LDO00_RS23925 Protein ID WP_001402821.1
Coordinates 4918593..4918895 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO00_RS23905 (4913901) 4913901..4915283 - 1383 WP_023155808.1 restriction endonuclease subunit S -
LDO00_RS23910 (4915273) 4915273..4916892 - 1620 WP_001029742.1 class I SAM-dependent DNA methyltransferase -
LDO00_RS23915 (4916956) 4916956..4918122 - 1167 WP_001672354.1 restriction endonuclease -
LDO00_RS23920 (4918319) 4918319..4918606 + 288 WP_001350372.1 BrnT family toxin Toxin
LDO00_RS23925 (4918593) 4918593..4918895 + 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
LDO00_RS23930 (4918929) 4918929..4919885 - 957 WP_001295525.1 GTPase -
LDO00_RS23935 (4919896) 4919896..4920099 - 204 WP_000467859.1 YbdD/YjiX family protein -
LDO00_RS23940 (4920149) 4920149..4922299 - 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
LDO00_RS23945 (4922692) 4922692..4923204 - 513 WP_001175456.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T38606 WP_001350372.1 NZ_AP024521:4918319-4918606 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T38606 NZ_AP024521:4918319-4918606 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTTACGGCATCGTGGTTATCCTGGTTGCGCATACCATCCGTTTCGAAAGTGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT38606 WP_001402821.1 NZ_AP024521:4918593-4918895 [Escherichia coli]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT38606 NZ_AP024521:4918593-4918895 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTATACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAGGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References