Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4684141..4684638 Replicon chromosome
Accession NZ_AP024521
Organism Escherichia coli strain E189

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LDO00_RS22735 Protein ID WP_001173343.1
Coordinates 4684465..4684638 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LDO00_RS22730 Protein ID WP_000398619.1
Coordinates 4684141..4684437 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO00_RS22710 (4680872) 4680872..4681591 - 720 WP_000611288.1 two-component system response regulator DcuR -
LDO00_RS22715 (4681588) 4681588..4683219 - 1632 WP_001216460.1 sensor histidine kinase -
LDO00_RS22720 (4683400) 4683400..4683630 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
LDO00_RS22725 (4683642) 4683642..4683914 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
LDO00_RS22730 (4684141) 4684141..4684437 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LDO00_RS22735 (4684465) 4684465..4684638 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LDO00_RS22740 (4684757) 4684757..4686274 - 1518 WP_023155344.1 lysine--tRNA ligase -
LDO00_RS22745 (4686511) 4686511..4687968 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T38604 WP_001173343.1 NZ_AP024521:4684465-4684638 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T38604 NZ_AP024521:4684465-4684638 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT38604 WP_000398619.1 NZ_AP024521:4684141-4684437 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT38604 NZ_AP024521:4684141-4684437 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References