Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3216743..3217397 Replicon chromosome
Accession NZ_AP024521
Organism Escherichia coli strain E189

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag LDO00_RS15485 Protein ID WP_000244772.1
Coordinates 3216743..3217150 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LDO00_RS15490 Protein ID WP_000354046.1
Coordinates 3217131..3217397 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO00_RS15465 (3212700) 3212700..3214433 - 1734 WP_023155905.1 single-stranded-DNA-specific exonuclease RecJ -
LDO00_RS15470 (3214439) 3214439..3215149 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LDO00_RS15475 (3215174) 3215174..3216070 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LDO00_RS15480 (3216182) 3216182..3216703 + 522 WP_001055874.1 flavodoxin FldB -
LDO00_RS15485 (3216743) 3216743..3217150 - 408 WP_000244772.1 protein YgfX Toxin
LDO00_RS15490 (3217131) 3217131..3217397 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LDO00_RS15495 (3217640) 3217640..3218620 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LDO00_RS15500 (3218697) 3218697..3219356 - 660 WP_000250274.1 hemolysin III family protein -
LDO00_RS15505 (3219520) 3219520..3219831 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
LDO00_RS15510 (3219876) 3219876..3221309 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
LDO00_RS15515 (3221366) 3221366..3222109 - 744 WP_023155906.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T38599 WP_000244772.1 NZ_AP024521:c3217150-3216743 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T38599 NZ_AP024521:c3217150-3216743 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT38599 WP_000354046.1 NZ_AP024521:c3217397-3217131 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT38599 NZ_AP024521:c3217397-3217131 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References