Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 85267..85868 Replicon plasmid pE148-2
Accession NZ_AP024520
Organism Escherichia coli strain E148

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag LDN98_RS25250 Protein ID WP_001216034.1
Coordinates 85267..85647 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LDN98_RS25255 Protein ID WP_001190712.1
Coordinates 85647..85868 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN98_RS25220 80367..80486 - 120 WP_071527722.1 ash family protein -
LDN98_RS25225 (NIAHE148_48270) 80708..82192 - 1485 WP_000124159.1 hypothetical protein -
LDN98_RS25230 (NIAHE148_48280) 82192..83385 - 1194 WP_166465177.1 terminase -
LDN98_RS25235 (NIAHE148_48290) 83471..83923 - 453 WP_001326849.1 late promoter-activating protein -
LDN98_RS25240 (NIAHE148_48300) 84012..85055 - 1044 WP_001561086.1 DUF968 domain-containing protein -
LDN98_RS25245 (NIAHE148_48310) 85083..85262 - 180 WP_000113018.1 hypothetical protein -
LDN98_RS25250 (NIAHE148_48320) 85267..85647 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LDN98_RS25255 (NIAHE148_48330) 85647..85868 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LDN98_RS25260 (NIAHE148_48340) 86051..87607 + 1557 WP_166465176.1 type I restriction-modification system subunit M -
LDN98_RS25265 (NIAHE148_48350) 87604..88806 + 1203 WP_166465175.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..99051 99051


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T38581 WP_001216034.1 NZ_AP024520:c85647-85267 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T38581 NZ_AP024520:c85647-85267 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT38581 WP_001190712.1 NZ_AP024520:c85868-85647 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT38581 NZ_AP024520:c85868-85647 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References