Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 22464..23107 Replicon plasmid pE148-1
Accession NZ_AP024519
Organism Escherichia coli strain E148

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag LDN98_RS24365 Protein ID WP_001044768.1
Coordinates 22691..23107 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag LDN98_RS24360 Protein ID WP_001261287.1
Coordinates 22464..22694 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN98_RS24340 (17624) 17624..18712 + 1089 WP_000952217.1 transcriptional repressor PifC -
LDN98_RS24345 (18714) 18714..20939 + 2226 WP_000698737.1 P-loop NTPase fold protein -
LDN98_RS24350 (20989) 20989..21888 - 900 WP_000963206.1 nucleotide-binding protein -
LDN98_RS24355 (21878) 21878..22168 - 291 WP_000111771.1 hypothetical protein -
LDN98_RS24360 (22464) 22464..22694 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LDN98_RS24365 (22691) 22691..23107 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
LDN98_RS24370 (23269) 23269..25407 - 2139 WP_000350638.1 AAA family ATPase -
LDN98_RS24375 (25761) 25761..26018 + 258 WP_000343085.1 hypothetical protein -
LDN98_RS24380 (26018) 26018..26608 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ARR-3 / tet(A) / mph(A) - 1..100617 100617


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T38577 WP_001044768.1 NZ_AP024519:22691-23107 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T38577 NZ_AP024519:22691-23107 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT38577 WP_001261287.1 NZ_AP024519:22464-22694 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT38577 NZ_AP024519:22464-22694 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References