Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3810502..3811088 Replicon chromosome
Accession NZ_AP024518
Organism Escherichia coli strain E148

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag LDN98_RS18505 Protein ID WP_042014162.1
Coordinates 3810858..3811088 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LDN98_RS18500 Protein ID WP_000593555.1
Coordinates 3810502..3810861 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN98_RS18475 (3806634) 3806634..3807578 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
LDN98_RS18480 (3807575) 3807575..3808408 + 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
LDN98_RS18485 (3808408) 3808408..3809172 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
LDN98_RS18490 (3809169) 3809169..3809975 + 807 WP_155053606.1 nickel import ATP-binding protein NikE -
LDN98_RS18495 (3809981) 3809981..3810382 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LDN98_RS18500 (3810502) 3810502..3810861 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LDN98_RS18505 (3810858) 3810858..3811088 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
LDN98_RS18510 (3811192) 3811192..3812316 - 1125 WP_001314210.1 ABC transporter permease -
LDN98_RS18515 (3812316) 3812316..3815051 - 2736 WP_000149168.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T38571 WP_042014162.1 NZ_AP024518:c3811088-3810858 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T38571 NZ_AP024518:c3811088-3810858 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT38571 WP_000593555.1 NZ_AP024518:c3810861-3810502 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT38571 NZ_AP024518:c3810861-3810502 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References