Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3215479..3216133 Replicon chromosome
Accession NZ_AP024518
Organism Escherichia coli strain E148

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag LDN98_RS15495 Protein ID WP_000244772.1
Coordinates 3215479..3215886 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LDN98_RS15500 Protein ID WP_000354046.1
Coordinates 3215867..3216133 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN98_RS15475 (3211436) 3211436..3213169 - 1734 WP_023155905.1 single-stranded-DNA-specific exonuclease RecJ -
LDN98_RS15480 (3213175) 3213175..3213885 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LDN98_RS15485 (3213910) 3213910..3214806 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LDN98_RS15490 (3214918) 3214918..3215439 + 522 WP_001055874.1 flavodoxin FldB -
LDN98_RS15495 (3215479) 3215479..3215886 - 408 WP_000244772.1 protein YgfX Toxin
LDN98_RS15500 (3215867) 3215867..3216133 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LDN98_RS15505 (3216376) 3216376..3217356 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LDN98_RS15510 (3217433) 3217433..3218092 - 660 WP_000250274.1 hemolysin III family protein -
LDN98_RS15515 (3218256) 3218256..3218567 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
LDN98_RS15520 (3218612) 3218612..3220045 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
LDN98_RS15525 (3220102) 3220102..3220845 - 744 WP_023155906.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T38569 WP_000244772.1 NZ_AP024518:c3215886-3215479 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T38569 NZ_AP024518:c3215886-3215479 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT38569 WP_000354046.1 NZ_AP024518:c3216133-3215867 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT38569 NZ_AP024518:c3216133-3215867 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References