Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2742032..2742657 Replicon chromosome
Accession NZ_AP024518
Organism Escherichia coli strain E148

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag LDN98_RS13310 Protein ID WP_000911329.1
Coordinates 2742032..2742430 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag LDN98_RS13315 Protein ID WP_000450524.1
Coordinates 2742430..2742657 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN98_RS13290 (2737910) 2737910..2738110 + 201 WP_000383836.1 YpfN family protein -
LDN98_RS13295 (2738220) 2738220..2738918 - 699 WP_000679823.1 esterase -
LDN98_RS13300 (2738992) 2738992..2741007 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
LDN98_RS13305 (2741022) 2741022..2741885 - 864 WP_001267498.1 neutral zinc metallopeptidase -
LDN98_RS13310 (2742032) 2742032..2742430 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
LDN98_RS13315 (2742430) 2742430..2742657 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
LDN98_RS13320 (2742811) 2742811..2743524 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
LDN98_RS13325 (2743737) 2743737..2744771 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
LDN98_RS13330 (2744788) 2744788..2745666 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
LDN98_RS13335 (2745812) 2745812..2746384 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
LDN98_RS13340 (2746384) 2746384..2746854 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T38567 WP_000911329.1 NZ_AP024518:c2742430-2742032 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T38567 NZ_AP024518:c2742430-2742032 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT38567 WP_000450524.1 NZ_AP024518:c2742657-2742430 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT38567 NZ_AP024518:c2742657-2742430 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References