Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 547366..547984 Replicon chromosome
Accession NZ_AP024518
Organism Escherichia coli strain E148

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LDN98_RS02645 Protein ID WP_001291435.1
Coordinates 547366..547584 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LDN98_RS02650 Protein ID WP_000344800.1
Coordinates 547610..547984 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN98_RS02610 (542656) 542656..543228 + 573 WP_000779826.1 YbaY family lipoprotein -
LDN98_RS02615 (543259) 543259..543570 - 312 WP_000409911.1 MGMT family protein -
LDN98_RS02625 (543949) 543949..544302 + 354 WP_000878140.1 DUF1428 domain-containing protein -
LDN98_RS02630 (544344) 544344..545894 - 1551 WP_024166621.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LDN98_RS02635 (546058) 546058..546528 - 471 WP_000136192.1 YlaC family protein -
LDN98_RS02640 (546644) 546644..547195 - 552 WP_000102556.1 maltose O-acetyltransferase -
LDN98_RS02645 (547366) 547366..547584 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LDN98_RS02650 (547610) 547610..547984 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LDN98_RS02655 (548530) 548530..551679 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LDN98_RS02660 (551702) 551702..552895 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T38553 WP_001291435.1 NZ_AP024518:c547584-547366 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T38553 NZ_AP024518:c547584-547366 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT38553 WP_000344800.1 NZ_AP024518:c547984-547610 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT38553 NZ_AP024518:c547984-547610 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References