Detailed information of TA system TA038550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/LOTUS_5_Limkain_b1-MazE
• Location: 2734645..2735421
• Replicon: chromosome
• Accession: NZ_AP024515
• Organism: Nitrosospira sp. NRS527

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KJS40_RS12215
• Protein ID: WP_213538104.1
• Coordinates: 2734645..2735052 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KJS40_RS12220
• Protein ID: WP_213538106.1
• Coordinates: 2735179..2735421 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJS40_RS12185 (NNRS527_02584)	2729883..2730989	+	1107	WP_213538093.1	glycosyl transferase	-
KJS40_RS12190 (NNRS527_02585)	2731250..2732509	+	1260	WP_213538095.1	metallophosphoesterase family protein	-
KJS40_RS12195	2732646..2732975	-	330	WP_213538097.1	hypothetical protein	-
KJS40_RS12200 (NNRS527_02586)	2732966..2733826	+	861	WP_213538099.1	sulfite exporter TauE/SafE family protein	-
KJS40_RS12205 (NNRS527_02587)	2733823..2734140	+	318	WP_213538101.1	DUF1634 domain-containing protein	-
KJS40_RS12210 (NNRS527_02588)	2734370..2734648	+	279	WP_213538102.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KJS40_RS12215 (NNRS527_02589)	2734645..2735052	+	408	WP_213538104.1	type II toxin-antitoxin system VapC family toxin	Toxin
KJS40_RS12220 (NNRS527_02590)	2735179..2735421	+	243	WP_213538106.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KJS40_RS12225 (NNRS527_02591)	2735421..2735759	+	339	WP_213538108.1	endoribonuclease MazF	-
KJS40_RS12230 (NNRS527_02592)	2735942..2736868	+	927	WP_213538110.1	glycosyltransferase family 2 protein	-
KJS40_RS12235 (NNRS527_02593)	2736937..2737830	+	894	WP_213538112.1	NAD-dependent epimerase/dehydratase family protein	-
KJS40_RS12240 (NNRS527_02594)	2737940..2738374	+	435	WP_213538114.1	alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB	-
KJS40_RS12245 (NNRS527_02595)	2738462..2740009	-	1548	WP_213538116.1	HAMP domain-containing sensor histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-120)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14969.31 Da
• Isoelectric Point: 8.0478

>T38550 WP_213538104.1 NZ_AP024515:2734645-2735052 [Nitrosospira sp. NRS527]
MILLDTVVLSELRKRQLSPEVVRWIAGFQDSDLFLSVVSIGEIERGIEKKRKNDPNFAASLTRWIEDLLRLYGDRVLPVT
PAIARCWGMLSARIGNDSADLLIAATALTHGLQVATRNTRHFTPTGVGVINPFDA

Nucleotide

• Length: 408 bp

>T38550 NZ_AP024515:2734645-2735052 [Nitrosospira sp. NRS527]
ATGATTCTGCTGGATACGGTTGTTCTTTCGGAGCTGCGAAAGCGCCAGCTTTCGCCAGAGGTGGTGCGCTGGATCGCCGG
CTTTCAGGATAGCGATTTGTTTCTCAGCGTAGTCAGTATCGGCGAAATCGAACGCGGCATTGAGAAGAAGCGAAAAAACG
ACCCTAATTTTGCCGCGTCACTCACGCGATGGATTGAAGATTTATTGCGTCTTTACGGTGATCGGGTACTACCTGTCACA
CCTGCTATAGCTCGCTGCTGGGGTATGCTCTCTGCCCGAATAGGAAACGATAGCGCCGATTTACTGATTGCAGCCACAGC
GTTAACCCATGGATTGCAGGTCGCTACAAGGAATACCCGGCACTTTACGCCTACTGGCGTTGGCGTGATCAATCCATTCG
ACGCCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8962.36 Da
• Isoelectric Point: 4.8047

>AT38550 WP_213538106.1 NZ_AP024515:2735179-2735421 [Nitrosospira sp. NRS527]
MRVIVKKWGNSASVRIPAAIMEAARLNLEDPVDIREEDGRIVIEPIHKNKYDLAQMLADITPENLHAEVDFGTPVGKELL

Nucleotide

• Length: 243 bp

>AT38550 NZ_AP024515:2735179-2735421 [Nitrosospira sp. NRS527]
ATGCGCGTAATCGTTAAAAAGTGGGGCAATAGCGCATCGGTTCGCATCCCTGCTGCGATCATGGAAGCTGCCCGCTTAAA
CTTGGAAGATCCGGTGGATATTCGTGAAGAAGATGGTCGCATCGTCATCGAACCGATTCACAAAAACAAATACGACCTTG
CTCAGATGCTTGCCGATATAACACCGGAAAATCTGCATGCCGAAGTTGATTTCGGTACACCGGTCGGCAAAGAGCTTTTG
TAA