Detailed information of TA system TA038540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 147920..148425
• Replicon: chromosome
• Accession: NZ_AP024513
• Organism: Pseudomonas aeruginosa isolate Pa12

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: KJS76_RS00665
• Protein ID: WP_003083773.1
• Coordinates: 147920..148201 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: KJS76_RS00670
• Protein ID: WP_003083775.1
• Coordinates: 148198..148425 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJS76_RS00640 (RVB2_01270)	143171..144520	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
KJS76_RS00645 (RVB2_01280)	144569..145255	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
KJS76_RS00650 (RVB2_01290)	145356..146090	+	735	WP_003117208.1	GntR family transcriptional regulator	-
KJS76_RS00655 (RVB2_01300)	146270..146680	+	411	WP_003110659.1	aegerolysin family protein	-
KJS76_RS00660 (RVB2_01310)	146712..147620	-	909	WP_003083769.1	LysR family transcriptional regulator	-
KJS76_RS00665 (RVB2_01320)	147920..148201	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
KJS76_RS00670 (RVB2_01330)	148198..148425	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
KJS76_RS00675 (RVB2_01340)	148600..149220	-	621	WP_003101226.1	hypothetical protein	-
KJS76_RS00680 (RVB2_01350)	149321..149821	+	501	WP_003162763.1	LEA type 2 family protein	-
KJS76_RS00685 (RVB2_01360)	149894..150235	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
KJS76_RS00690 (RVB2_01370)	150317..151744	-	1428	WP_003083784.1	GABA permease	-
KJS76_RS00695 (RVB2_01380)	151913..153406	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T38540 WP_003083773.1 NZ_AP024513:c148201-147920 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T38540 NZ_AP024513:c148201-147920 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT38540 WP_003083775.1 NZ_AP024513:c148425-148198 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT38540 NZ_AP024513:c148425-148198 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9