Detailed information of TA system TA038538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2815299..2815828
• Replicon: chromosome
• Accession: NZ_AP024511
• Organism: Staphylococcus aureus isolate 2007-13

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KJS82_RS13940
• Protein ID: WP_000621175.1
• Coordinates: 2815466..2815828 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KJS82_RS13935
• Protein ID: WP_000948331.1
• Coordinates: 2815299..2815469 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJS82_RS13905 (2810336)	2810336..2810896	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KJS82_RS13910 (2811104)	2811104..2811583	+	480	WP_001287087.1	hypothetical protein	-
KJS82_RS13915 (2811576)	2811576..2813159	+	1584	WP_001669049.1	PH domain-containing protein	-
KJS82_RS13920 (2813146)	2813146..2813637	+	492	WP_001205912.1	PH domain-containing protein	-
KJS82_RS13925 (2813641)	2813641..2814000	+	360	WP_000581197.1	holo-ACP synthase	-
KJS82_RS13930 (2814066)	2814066..2815214	+	1149	WP_001281154.1	alanine racemase	-
KJS82_RS13935 (2815299)	2815299..2815469	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KJS82_RS13940 (2815466)	2815466..2815828	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KJS82_RS13945 (2816177)	2816177..2817178	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KJS82_RS13950 (2817297)	2817297..2817623	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KJS82_RS13955 (2817625)	2817625..2818104	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KJS82_RS13960 (2818079)	2818079..2818849	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T38538 WP_000621175.1 NZ_AP024511:2815466-2815828 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T38538 NZ_AP024511:2815466-2815828 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT38538 WP_000948331.1 NZ_AP024511:2815299-2815469 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT38538 NZ_AP024511:2815299-2815469 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1