Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 252881..253523 Replicon chromosome
Accession NZ_AP024504
Organism Bacillus cereus strain HT18

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag WHT_RS01415 Protein ID WP_000635963.1
Coordinates 253173..253523 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag WHT_RS01410 Protein ID WP_000004570.1
Coordinates 252881..253168 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WHT_RS01385 (WHT_c02690) 248198..249160 + 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -
WHT_RS01390 (WHT_c02700) 249153..249725 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
WHT_RS01395 (WHT_c02710) 249819..250178 + 360 WP_000583416.1 holo-ACP synthase -
WHT_RS01400 (WHT_c02720) 250335..251285 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
WHT_RS01405 (WHT_c02730) 251403..252572 + 1170 WP_000390615.1 alanine racemase -
WHT_RS01410 (WHT_c02740) 252881..253168 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
WHT_RS01415 (WHT_c02750) 253173..253523 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
WHT_RS01420 (WHT_c02760) 253591..255759 + 2169 WP_000426242.1 Tex family protein -
WHT_RS01425 255817..255933 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
WHT_RS01430 (WHT_c02770) 256129..256587 + 459 WP_000344234.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T38515 WP_000635963.1 NZ_AP024504:253173-253523 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T38515 NZ_AP024504:253173-253523 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT38515 WP_000004570.1 NZ_AP024504:252881-253168 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT38515 NZ_AP024504:252881-253168 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References