Detailed information of TA system TA038508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2433900..2434489
• Replicon: chromosome
• Accession: NZ_AP024503
• Organism: Pseudomonas protegens strain Go58

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7V8EXE5
• Locus tag: PproGo58_RS10995
• Protein ID: WP_019094202.1
• Coordinates: 2434238..2434489 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PproGo58_RS10990
• Protein ID: WP_207167424.1
• Coordinates: 2433900..2434241 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PproGo58_RS10965	2429306..2430253	-	948	WP_102864139.1	nucleoside-specific channel-forming protein Tsx	-
PproGo58_RS10970	2430504..2430869	+	366	WP_207167421.1	DUF4440 domain-containing protein	-
PproGo58_RS10975	2430915..2431949	+	1035	WP_060838607.1	selenide, water dikinase SelD	-
PproGo58_RS10980	2431949..2433049	+	1101	WP_207167422.1	tRNA 2-selenouridine(34) synthase MnmH	-
PproGo58_RS10985	2433108..2433449	-	342	WP_207167423.1	DUF3077 domain-containing protein	-
PproGo58_RS10990	2433900..2434241	-	342	WP_207167424.1	toxin-antitoxin system HicB family antitoxin	Antitoxin
PproGo58_RS10995	2434238..2434489	-	252	WP_019094202.1	type II toxin-antitoxin system HicA family toxin	Toxin
PproGo58_RS11000	2434576..2435631	-	1056	WP_102864153.1	permease	-
PproGo58_RS11005	2436023..2436799	-	777	WP_060838611.1	IclR family transcriptional regulator	-
PproGo58_RS11010	2436970..2437734	+	765	WP_207167425.1	transporter substrate-binding domain-containing protein	-
PproGo58_RS11015	2437795..2438451	+	657	WP_011060581.1	amino acid ABC transporter permease	-
PproGo58_RS11020	2438448..2439194	+	747	WP_207167426.1	ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9239.70 Da
• Isoelectric Point: 10.4707

>T38508 WP_019094202.1 NZ_AP024503:c2434489-2434238 [Pseudomonas protegens]
VSKNDKLLAKLLNDRGTFSWQELVTLLKRLGYTPLEGSGSRVKFDNGDPGAMINLHKPHPGNELKAYIRRQVIEQLKSGG
LIP

Nucleotide

• Length: 252 bp

>T38508 NZ_AP024503:c2434489-2434238 [Pseudomonas protegens]
GTGTCCAAGAACGATAAGCTTCTCGCCAAGTTACTCAACGACCGGGGGACCTTTTCCTGGCAGGAACTGGTCACTCTCCT
AAAGCGCCTGGGCTACACACCGTTGGAGGGCTCAGGCAGTCGGGTAAAATTCGACAATGGCGATCCAGGCGCAATGATCA
ACCTGCACAAACCGCACCCCGGTAATGAGCTTAAGGCCTACATACGCCGCCAAGTGATCGAGCAACTGAAATCGGGAGGT
CTCATACCGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12456.88 Da
• Isoelectric Point: 5.0710

>AT38508 WP_207167424.1 NZ_AP024503:c2434241-2433900 [Pseudomonas protegens]
VNNLLQYKGYFGSIEVSTEDNCLFGKLQFIRALVSYEGQTVAGLEAAFKEAVDDYLDTCEQSGTAPEIPCKGSFNVRVGH
DLHLAAAVAATRQNVSLNDLTRQALNEYLQHHH

Nucleotide

• Length: 342 bp

>AT38508 NZ_AP024503:c2434241-2433900 [Pseudomonas protegens]
GTGAACAACTTACTGCAGTACAAGGGCTATTTCGGCTCTATCGAAGTCAGTACCGAGGACAATTGCCTCTTTGGCAAACT
TCAGTTCATAAGGGCCCTGGTTAGCTATGAAGGGCAGACCGTGGCTGGACTCGAAGCGGCCTTCAAAGAGGCAGTAGACG
ACTATCTGGATACCTGTGAACAATCGGGTACAGCACCCGAAATCCCTTGCAAAGGCTCGTTCAACGTTCGGGTAGGCCAC
GACCTTCATCTAGCGGCTGCCGTGGCCGCCACCCGGCAGAATGTATCGTTGAACGATCTGACCCGTCAGGCCCTGAACGA
ATACCTGCAACATCACCACTGA

Structures

Toxin

• AlphaFold DB: A0A7V8EXE5