Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 483203..483840 Replicon chromosome
Accession NZ_AP024501
Organism Bacillus velezensis strain AD-3

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BVAD3_RS02425 Protein ID WP_003156187.1
Coordinates 483490..483840 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BVAD3_RS02420 Protein ID WP_003156188.1
Coordinates 483203..483484 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVAD3_RS02400 479568..480167 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
BVAD3_RS02405 480260..480625 + 366 WP_014304402.1 holo-ACP synthase -
BVAD3_RS02410 480790..481797 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
BVAD3_RS02415 481914..483083 + 1170 WP_014416966.1 alanine racemase -
BVAD3_RS02420 483203..483484 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BVAD3_RS02425 483490..483840 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BVAD3_RS02430 483958..484779 + 822 WP_003156182.1 STAS domain-containing protein -
BVAD3_RS02435 484784..485149 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
BVAD3_RS02440 485152..485553 + 402 WP_003156178.1 anti-sigma regulatory factor -
BVAD3_RS02445 485565..486572 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
BVAD3_RS02450 486636..486965 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BVAD3_RS02455 486962..487444 + 483 WP_007609591.1 anti-sigma B factor RsbW -
BVAD3_RS02460 487410..488198 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
BVAD3_RS02465 488198..488800 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T38503 WP_003156187.1 NZ_AP024501:483490-483840 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T38503 NZ_AP024501:483490-483840 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT38503 WP_003156188.1 NZ_AP024501:483203-483484 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT38503 NZ_AP024501:483203-483484 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References