Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 93986..94512 Replicon plasmid pEN30L
Accession NZ_AP024500
Organism Enterobacter asburiae strain En30

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag R2TS_RS23745 Protein ID WP_000323025.1
Coordinates 94225..94512 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag R2TS_RS23740 Protein ID WP_000534858.1
Coordinates 93986..94225 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R2TS_RS23725 90314..91552 + 1239 WP_000219087.1 IS110 family transposase -
R2TS_RS23730 92028..92600 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
R2TS_RS23735 92800..93723 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
R2TS_RS23740 93986..94225 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
R2TS_RS23745 94225..94512 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
R2TS_RS23750 94584..94742 + 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
R2TS_RS23755 95953..96156 - 204 WP_001015183.1 hypothetical protein -
R2TS_RS23760 96203..96553 - 351 WP_000743059.1 hypothetical protein -
R2TS_RS23765 96613..97215 - 603 WP_012695474.1 hypothetical protein -
R2TS_RS23770 97311..98255 - 945 WP_000778029.1 DUF5417 domain-containing protein -
R2TS_RS23775 98766..98942 + 177 WP_001371930.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA2 / tet(D) / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / blaTEM-1B - 1..284167 284167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T38501 WP_000323025.1 NZ_AP024500:94225-94512 [Enterobacter asburiae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T38501 NZ_AP024500:94225-94512 [Enterobacter asburiae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT38501 WP_000534858.1 NZ_AP024500:93986-94225 [Enterobacter asburiae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT38501 NZ_AP024500:93986-94225 [Enterobacter asburiae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References