Detailed information of TA system TA038483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1658182..1658979
• Replicon: chromosome
• Accession: NZ_AP024498
• Organism: Enterobacter asburiae strain En30

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: R2TS_RS07845
• Protein ID: WP_172421424.1
• Coordinates: 1658479..1658979 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: R2TS_RS07840
• Protein ID: WP_008499829.1
• Coordinates: 1658182..1658451 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R2TS_RS07810	1653809..1654252	-	444	WP_032658095.1	helix-turn-helix transcriptional regulator	-
R2TS_RS07815	1654405..1655646	+	1242	WP_182071674.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
R2TS_RS07820	1655680..1655907	-	228	WP_008499832.1	YccJ family protein	-
R2TS_RS07825	1655928..1656524	-	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
R2TS_RS07830	1656915..1657085	+	171	WP_023311077.1	general stress protein	-
R2TS_RS07835	1657203..1658120	+	918	WP_033145161.1	DMT family transporter	-
R2TS_RS07840	1658182..1658451	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
R2TS_RS07845	1658479..1658979	+	501	WP_172421424.1	GNAT family N-acetyltransferase	Toxin
R2TS_RS07850	1659053..1660375	-	1323	WP_182071673.1	pyrimidine utilization transport protein G	-
R2TS_RS07855	1660397..1660891	-	495	WP_032658088.1	pyrimidine utilization flavin reductase protein F	-
R2TS_RS07860	1660901..1661491	-	591	WP_047061542.1	malonic semialdehyde reductase	-
R2TS_RS07865	1661501..1662301	-	801	WP_182071672.1	pyrimidine utilization protein D	-
R2TS_RS07870	1662309..1662695	-	387	WP_010429508.1	pyrimidine utilization protein C	-
R2TS_RS07875	1662707..1663396	-	690	WP_182071671.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18969.67 Da
• Isoelectric Point: 7.9145

>T38483 WP_172421424.1 NZ_AP024498:1658479-1658979 [Enterobacter asburiae]
MLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQRRIPYINV
PSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTAENSHSLFFPTKSIE
RLFEQE

Nucleotide

• Length: 501 bp

>T38483 NZ_AP024498:1658479-1658979 [Enterobacter asburiae]
ATGTTATCAGAGGGAACCGATTATGACTTCGAGGATTTTGATTGTGGCGAACCCTCGCTAAATGCCTTCCTTGCTGAACA
TCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATCGTCCGCGAGTCCTGGGTTATT
ACACTTTATCGGGAAGCTGTTTTGAAAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGCCGAATCCCTTATATCAACGTA
CCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGGAACAACGCTCGTTGCGCATGC
TATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATGCCCTTAACGAGCGGGCAAAAC
GATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGCTGAAAACAGCCATTCTTTGTTCTTCCCTACAAAATCTATCGAA
CGGCTGTTTGAGCAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT38483 WP_008499829.1 NZ_AP024498:1658182-1658451 [Enterobacter asburiae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT38483 NZ_AP024498:1658182-1658451 [Enterobacter asburiae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGAATGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: W7PG43