Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1034646..1035266 Replicon chromosome
Accession NZ_AP024498
Organism Enterobacter asburiae strain En30

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag R2TS_RS04845 Protein ID WP_008499287.1
Coordinates 1034646..1034864 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag R2TS_RS04850 Protein ID WP_008499288.1
Coordinates 1034892..1035266 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R2TS_RS04815 1030660..1030920 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
R2TS_RS04820 1030923..1031063 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
R2TS_RS04825 1031060..1031770 - 711 WP_182071796.1 GNAT family N-acetyltransferase -
R2TS_RS04830 1031872..1033332 + 1461 WP_182071794.1 PLP-dependent aminotransferase family protein -
R2TS_RS04835 1033304..1033771 - 468 WP_045400719.1 membrane protein -
R2TS_RS04840 1033889..1034440 - 552 WP_182071792.1 maltose O-acetyltransferase -
R2TS_RS04845 1034646..1034864 - 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
R2TS_RS04850 1034892..1035266 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
R2TS_RS04855 1035778..1038924 - 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit -
R2TS_RS04860 1038947..1040140 - 1194 WP_029741963.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T38482 WP_008499287.1 NZ_AP024498:c1034864-1034646 [Enterobacter asburiae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T38482 NZ_AP024498:c1034864-1034646 [Enterobacter asburiae]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTTGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAACTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT38482 WP_008499288.1 NZ_AP024498:c1035266-1034892 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT38482 NZ_AP024498:c1035266-1034892 [Enterobacter asburiae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References