Detailed information of TA system TA038481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 592541..593117
• Replicon: chromosome
• Accession: NZ_AP024498
• Organism: Enterobacter asburiae strain En30

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A4R0G892
• Locus tag: R2TS_RS02765
• Protein ID: WP_010427219.1
• Coordinates: 592541..592828 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: R2TS_RS02770
• Protein ID: WP_048976412.1
• Coordinates: 592815..593117 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R2TS_RS02740	587657..588352	+	696	WP_182072048.1	winged helix-turn-helix domain-containing protein	-
R2TS_RS02745	588502..588972	+	471	WP_033144545.1	MarR family transcriptional regulator	-
R2TS_RS02750	588969..590036	+	1068	WP_127353288.1	HlyD family secretion protein	-
R2TS_RS02755	590026..591102	+	1077	WP_023310256.1	DUF2955 domain-containing protein	-
R2TS_RS02760	591099..592370	-	1272	WP_039260963.1	DUF445 domain-containing protein	-
R2TS_RS02765	592541..592828	+	288	WP_010427219.1	BrnT family toxin	Toxin
R2TS_RS02770	592815..593117	+	303	WP_048976412.1	BrnA antitoxin family protein	Antitoxin
R2TS_RS02775	593148..593786	-	639	WP_182072050.1	LysE family translocator	-
R2TS_RS02780	593834..594577	-	744	WP_182072052.1	AraC family transcriptional regulator	-
R2TS_RS02785	594729..596099	+	1371	WP_182072054.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
R2TS_RS02790	596144..596464	-	321	WP_182072056.1	hypothetical protein	-
R2TS_RS02795	596464..597009	-	546	WP_023310269.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11174.64 Da
• Isoelectric Point: 6.7609

>T38481 WP_010427219.1 NZ_AP024498:592541-592828 [Enterobacter asburiae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T38481 NZ_AP024498:592541-592828 [Enterobacter asburiae]
ATGCCAACGGAGTTTGAATGGGATACCAACAAAGCGAAAAGCAATCTCATTAAGCATGGGATCCGCTTTGAAGAAGCTGT
TCTGGTGTTTGACGATCCTTACCATTTATCTTTGCAGGATCGTCATGAAAATGGAGAGTTCCGTTGGCAGACCATTGGCC
TTGTACATGGTCTAATTGTCATTATGGTCGCACACACGGTACGTTTTGAAAGTGGTGACGAAGTCATTCGAATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAGCCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11497.19 Da
• Isoelectric Point: 10.0592

>AT38481 WP_048976412.1 NZ_AP024498:592815-593117 [Enterobacter asburiae]
MSMVKHKRGSAPEMSARREAELKALAEKSEAEIDYSDIPAMEDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT38481 NZ_AP024498:592815-593117 [Enterobacter asburiae]
ATGAGCATGGTTAAACATAAACGCGGTAGTGCGCCTGAGATGAGTGCTCGCCGTGAGGCTGAGCTCAAAGCACTAGCCGA
AAAATCGGAAGCAGAGATTGATTACAGCGATATCCCTGCCATGGAAGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTCAAAACTCAGGCGTCTGTACGTATCGACGCTGACGTGATGGAGTGGCTTAAACGGCCTGGAAAAGGT
TACCAGACGAGGCTCAATGCCATTTTGCGTGAAGCTATGCTGCGCGATCAAAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A4R0G892