Detailed information of TA system TA038476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 1945309..1945862
• Replicon: chromosome
• Accession: NZ_AP024487
• Organism: MAG: Metallosphaera javensis (ex Sakai et al. 2022) isolate AS-7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QVC96_RS10905
• Protein ID: WP_287689258.1
• Coordinates: 1945309..1945605 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: QVC96_RS10910
• Protein ID: WP_238027066.1
• Coordinates: 1945602..1945862 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QVC96_RS10880 (MjAS7_2372)	1941085..1941711	-	627	WP_238027061.1	PIG-L family deacetylase	-
QVC96_RS10885 (MjAS7_2373)	1941746..1942786	-	1041	WP_238027063.1	HoxN/HupN/NixA family nickel/cobalt transporter	-
QVC96_RS10890 (MjAS7_2374)	1942944..1944107	-	1164	WP_287689257.1	glycosyltransferase	-
QVC96_RS10895 (MjAS7_2375)	1944296..1944784	+	489	WP_238027283.1	helix-turn-helix domain-containing protein	-
QVC96_RS10900 (MjAS7_2376)	1944756..1945292	+	537	WP_287688834.1	IS630 family transposase	-
QVC96_RS10905 (MjAS7_2377)	1945309..1945605	-	297	WP_287689258.1	type II toxin-antitoxin system VapC family toxin	Toxin
QVC96_RS10910 (MjAS7_2378)	1945602..1945862	-	261	WP_238027066.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
QVC96_RS10915 (MjAS7_2379)	1946022..1946654	-	633	WP_287689259.1	type 1 glutamine amidotransferase	-
QVC96_RS10920 (MjAS7_2380)	1946824..1947345	-	522	WP_287689260.1	PaREP1 family protein	-
QVC96_RS10925 (MjAS7_2381)	1947501..1947707	+	207	WP_287689261.1	hypothetical protein	-
QVC96_RS10930 (MjAS7_2382)	1947828..1948478	+	651	WP_287689262.1	cyclase family protein	-
QVC96_RS10935 (MjAS7_2383)	1948670..1949122	+	453	WP_287689263.1	peroxiredoxin	-
QVC96_RS10940 (MjAS7_2384)	1949198..1949617	+	420	WP_287689264.1	hypothetical protein	-
QVC96_RS10945 (MjAS7_2385)	1949633..1950259	+	627	WP_287689265.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-98)

Antitoxin

• SpoVT-AbrB domain (4-46)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11329.29 Da
• Isoelectric Point: 4.5238

>T38476 WP_287689258.1 NZ_AP024487:c1945605-1945309 [Metallosphaera javensis (ex Sakai et al. 2022)]
MIFLDANFLVYLNLNVDPVKRFYLKLLSSESLALDALVLDEVIYVSRKKYQVDFSDTVEFLDQLVLPYVTLLPITLREYE
VGKGIMLRYSIPPSDALH

Nucleotide

• Length: 297 bp

>T38476 NZ_AP024487:c1945605-1945309 [Metallosphaera javensis (ex Sakai et al. 2022)]
TTGATATTTCTGGACGCAAACTTTCTCGTATATCTTAACCTAAATGTGGATCCGGTAAAGAGGTTCTATCTGAAACTTCT
GTCCAGCGAGAGTCTTGCCCTGGACGCGCTTGTGCTGGATGAGGTTATTTACGTCTCAAGGAAAAAGTATCAGGTTGACT
TTAGCGACACCGTGGAGTTCCTGGATCAGCTCGTTCTACCCTATGTGACCCTGCTCCCCATTACCCTGAGGGAGTACGAG
GTGGGTAAGGGAATAATGCTAAGGTACTCCATTCCTCCCTCCGATGCCCTTCACTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9986.47 Da
• Isoelectric Point: 5.2813

>AT38476 WP_238027066.1 NZ_AP024487:c1945862-1945602 [Metallosphaera javensis (ex Sakai et al. 2022)]
MTIRVEVGKKGYLIIPKSVRDLLGINEGDTLELRVEDGKIILERERNVDFKELERKFEEHGRKIAYARKAQLGDLKGLSL
EEEFQD

Nucleotide

• Length: 261 bp

>AT38476 NZ_AP024487:c1945862-1945602 [Metallosphaera javensis (ex Sakai et al. 2022)]
TTGACCATCAGGGTGGAAGTGGGGAAGAAGGGGTACTTGATAATCCCCAAGAGTGTGAGGGATCTCCTGGGAATCAATGA
GGGTGACACCCTCGAACTGAGGGTCGAGGACGGAAAAATAATTCTGGAGAGGGAGAGGAACGTTGACTTTAAGGAACTTG
AGAGGAAATTTGAGGAACATGGCAGGAAGATAGCCTACGCAAGGAAGGCACAACTGGGAGACCTGAAGGGTCTCTCCCTG
GAAGAGGAGTTCCAAGATTGA