Detailed information of TA system TA038473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1383525..1384140
• Replicon: chromosome
• Accession: NZ_AP024487
• Organism: MAG: Metallosphaera javensis (ex Sakai et al. 2022) isolate AS-7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QVC96_RS08080
• Protein ID: WP_238026886.1
• Coordinates: 1383739..1384140 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: QVC96_RS08075
• Protein ID: WP_238026885.1
• Coordinates: 1383525..1383749 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QVC96_RS08045 (MjAS7_1687)	1379454..1380014	+	561	WP_287688846.1	hypothetical protein	-
QVC96_RS08050 (MjAS7_1688)	1380068..1380463	+	396	WP_287688847.1	PadR family transcriptional regulator	-
QVC96_RS08055 (MjAS7_1689)	1380460..1381377	+	918	WP_287688848.1	ABC transporter ATP-binding protein	-
QVC96_RS08060 (MjAS7_1690)	1381365..1382165	+	801	WP_287690456.1	ABC transporter permease	-
QVC96_RS08065 (MjAS7_1691)	1382279..1382782	-	504	WP_287688849.1	hypothetical protein	-
QVC96_RS08070 (MjAS7_1692)	1382977..1383162	+	186	WP_287688850.1	hypothetical protein	-
QVC96_RS08075 (MjAS7_1693)	1383525..1383749	+	225	WP_238026885.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
QVC96_RS08080 (MjAS7_1694)	1383739..1384140	+	402	WP_238026886.1	type II toxin-antitoxin system VapC family toxin	Toxin
QVC96_RS08085 (MjAS7_1695)	1384460..1384816	+	357	WP_287688851.1	DUF488 domain-containing protein	-
QVC96_RS08090 (MjAS7_1696)	1384836..1386314	-	1479	WP_238026888.1	ammonium transporter	-
QVC96_RS08095 (MjAS7_1697)	1386668..1388263	-	1596	WP_238026889.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• Post-segregation antitoxin CcdA (5-56)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15539.14 Da
• Isoelectric Point: 4.7915

>T38473 WP_238026886.1 NZ_AP024487:1383739-1384140 [Metallosphaera javensis (ex Sakai et al. 2022)]
MKDKYLFDTSALYPFIMELLKGKISFDFLSNIYILDLTVYEIGNVLWKDSKISNKIKDPVETMENFLNIIKKFNLISKID
YVKVLKIAIERDLTFYDSAYVYVAEENNLTLITYDEELLGKTKNAKKLDEIIG

Nucleotide

• Length: 402 bp

>T38473 NZ_AP024487:1383739-1384140 [Metallosphaera javensis (ex Sakai et al. 2022)]
ATGAAAGATAAATATCTCTTCGATACTTCAGCGTTATATCCGTTCATCATGGAATTACTAAAGGGAAAAATAAGTTTTGA
CTTCCTTTCTAACATTTACATACTGGATTTAACAGTTTATGAAATCGGTAACGTCTTATGGAAAGATAGTAAAATTAGCA
ATAAAATAAAAGACCCTGTAGAAACAATGGAAAATTTCCTAAATATAATAAAAAAATTCAATTTGATAAGCAAGATTGAC
TACGTTAAGGTATTAAAAATAGCAATTGAGAGGGACTTAACATTTTACGACAGTGCATACGTTTATGTAGCTGAAGAAAA
CAATCTCACTCTGATCACCTACGATGAGGAATTACTAGGAAAAACAAAAAACGCAAAGAAACTGGACGAAATCATAGGTT
AG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8861.28 Da
• Isoelectric Point: 5.3339

>AT38473 WP_238026885.1 NZ_AP024487:1383525-1383749 [Metallosphaera javensis (ex Sakai et al. 2022)]
MSDVISVRVKRQLKEKAEELGIDIRQVVEKALGEEIKKREEEEIRKSAEIIVENMKEVKIQEWIKLVRETRDER

Nucleotide

• Length: 225 bp

>AT38473 NZ_AP024487:1383525-1383749 [Metallosphaera javensis (ex Sakai et al. 2022)]
ATGTCTGACGTAATTTCTGTTAGAGTCAAAAGACAGTTAAAGGAGAAAGCCGAGGAATTAGGAATAGATATAAGACAGGT
AGTGGAAAAAGCCCTGGGAGAGGAAATAAAGAAAAGAGAAGAAGAGGAAATAAGGAAATCAGCAGAAATAATAGTGGAAA
ACATGAAGGAAGTTAAAATACAAGAGTGGATAAAACTCGTGAGAGAAACGAGAGATGAAAGATAA