Detailed information of TA system TA038469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2282396..2282992
• Replicon: chromosome
• Accession: NZ_AP024470
• Organism: Asaccharobacter celatus DSM 18785

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: ADLECEL_RS09480
• Protein ID: WP_117284338.1
• Coordinates: 2282396..2282692 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ADLECEL_RS09485
• Protein ID: WP_117284337.1
• Coordinates: 2282705..2282992 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ADLECEL_RS09450	2277785..2278783	+	999	WP_117284343.1	carbon-nitrogen hydrolase family protein	-
ADLECEL_RS09455	2278809..2279483	-	675	WP_117284347.1	TetR/AcrR family transcriptional regulator	-
ADLECEL_RS09460	2279662..2280624	+	963	WP_117284342.1	hypothetical protein	-
ADLECEL_RS09465	2280942..2281421	+	480	WP_117284341.1	peptide-methionine (S)-S-oxide reductase	-
ADLECEL_RS09470	2281633..2282040	+	408	WP_117284340.1	hypothetical protein	-
ADLECEL_RS09475	2282037..2282303	+	267	WP_117284339.1	hypothetical protein	-
ADLECEL_RS09480	2282396..2282692	+	297	WP_117284338.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ADLECEL_RS09485	2282705..2282992	+	288	WP_117284337.1	putative addiction module antidote protein	Antitoxin
ADLECEL_RS09490	2283054..2284658	-	1605	WP_117284336.1	glutamine-hydrolyzing GMP synthase	-
ADLECEL_RS09495	2284861..2285736	-	876	WP_117284335.1	tyrosine-protein phosphatase	-
ADLECEL_RS09500	2285911..2286288	-	378	WP_117284334.1	hypothetical protein	-
ADLECEL_RS09505	2286597..2287001	+	405	WP_181965280.1	metalloregulator ArsR/SmtB family transcription factor	-
ADLECEL_RS09510	2287072..2287299	+	228	WP_117284333.1	cation transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-89)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11438.38 Da
• Isoelectric Point: 10.4470

>T38469 WP_117284338.1 NZ_AP024470:2282396-2282692 [Asaccharobacter celatus DSM 18785]
MITVLQTTEFVKWLKRLKDAEARARILVRIRRLSLTENFGDVKPVGDCVYEMRVEHGPGYRLYYARRDNALVLLLIGGDK
SSQQRDIAKAKKLNLEYR

Nucleotide

• Length: 297 bp

>T38469 NZ_AP024470:2282396-2282692 [Asaccharobacter celatus DSM 18785]
ATGATAACCGTCCTGCAAACAACCGAGTTCGTGAAATGGCTCAAACGACTCAAAGATGCTGAGGCGCGAGCGCGTATTCT
CGTGAGAATACGGCGCCTTTCACTAACGGAGAATTTTGGAGACGTTAAGCCAGTTGGCGACTGCGTTTATGAGATGCGCG
TCGAGCACGGGCCTGGATATCGTCTCTATTACGCAAGACGGGACAATGCGTTGGTCTTGCTCCTCATCGGTGGAGACAAA
TCATCGCAACAGCGAGACATCGCGAAAGCAAAAAAGCTCAACCTCGAGTACAGGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 9909.17 Da
• Isoelectric Point: 4.5105

>AT38469 WP_117284337.1 NZ_AP024470:2282705-2282992 [Asaccharobacter celatus DSM 18785]
MKKATTWDAADYLETEADIAAYLNAAFEDGDTSVIAAALGDVARAKGMTQLSKETGITRDGLYKALSPAGNPSLDTVQKV
VRAFGLKLDVSAVSA

Nucleotide

• Length: 288 bp

>AT38469 NZ_AP024470:2282705-2282992 [Asaccharobacter celatus DSM 18785]
ATGAAGAAAGCGACAACCTGGGACGCAGCCGACTATCTCGAAACGGAAGCCGACATTGCGGCCTATCTAAATGCCGCCTT
TGAAGACGGCGACACCTCCGTAATCGCCGCCGCCCTTGGCGACGTCGCCCGGGCAAAGGGAATGACGCAGCTCTCAAAGG
AAACCGGCATCACCAGAGACGGTCTTTACAAGGCGTTATCACCGGCAGGGAATCCGTCGCTTGATACCGTACAGAAAGTC
GTGAGGGCCTTTGGACTCAAACTCGACGTTTCGGCAGTCTCGGCATAA