Detailed information of TA system TA038435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 3993457..3994004
• Replicon: chromosome
• Accession: NZ_AP024354
• Organism: Pseudomonas alcaligenes strain KAM426

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JUH08_RS19275
• Protein ID: WP_061194819.1
• Coordinates: 3993729..3994004 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JUH08_RS19270
• Protein ID: WP_061194820.1
• Coordinates: 3993457..3993729 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JUH08_RS19240	3990227..3990745	-	519	WP_203792471.1	peptide-methionine (S)-S-oxide reductase MsrA	-
JUH08_RS19245	3990858..3991082	-	225	Protein_3777	helix-turn-helix domain-containing protein	-
JUH08_RS19250	3991368..3992138	+	771	WP_061198833.1	DUF2807 domain-containing protein	-
JUH08_RS19255	3992158..3992427	+	270	WP_061194823.1	hypothetical protein	-
JUH08_RS19260	3992443..3992892	+	450	WP_061199210.1	hypothetical protein	-
JUH08_RS19265	3992889..3993473	+	585	WP_061194821.1	hypothetical protein	-
JUH08_RS19270	3993457..3993729	+	273	WP_061194820.1	ribbon-helix-helix domain-containing protein	Antitoxin
JUH08_RS19275	3993729..3994004	+	276	WP_061194819.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JUH08_RS19280	3994006..3994833	+	828	WP_061194818.1	hypothetical protein	-
JUH08_RS19285	3994847..3995089	+	243	WP_203787088.1	hypothetical protein	-
JUH08_RS19290	3995144..3995389	+	246	WP_061194816.1	hypothetical protein	-
JUH08_RS19295	3995564..3996766	+	1203	WP_203788698.1	hypothetical protein	-
JUH08_RS19300	3996751..3998292	+	1542	WP_203788700.1	Hsp70 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	sul1 / blaIMP-1	-	3786007..4015023	229016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10673.22 Da
• Isoelectric Point: 7.2020

>T38435 WP_061194819.1 NZ_AP024354:3993729-3994004 [Pseudomonas alcaligenes]
MELKWTSKALSDLARLYEFLAAVNQPAAARTVQQLTAAPTTLLTNPRIGERLEEFDPRDVRRILVGHYEMRYEIADSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T38435 NZ_AP024354:3993729-3994004 [Pseudomonas alcaligenes]
ATGGAGTTGAAGTGGACGAGCAAGGCCCTATCCGATCTGGCCCGCCTCTATGAGTTCCTTGCAGCGGTGAACCAGCCCGC
TGCGGCGCGCACAGTGCAGCAGCTCACCGCAGCGCCGACGACGCTGCTGACCAACCCGCGCATCGGCGAGCGGCTGGAAG
AGTTCGATCCGCGCGACGTGCGACGGATTCTGGTCGGGCACTACGAGATGCGCTACGAAATAGCAGACTCCACCATCTAC
CTGCTGCGCCTGTGGCATACACGCGAGGATCGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10054.25 Da
• Isoelectric Point: 4.8616

>AT38435 WP_061194820.1 NZ_AP024354:3993457-3993729 [Pseudomonas alcaligenes]
MAATAKTRSVTAHVPVELAERVDEIAERLERSKNWIVKQALSAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWADSL
STDTPLPVPH

Nucleotide

• Length: 273 bp

>AT38435 NZ_AP024354:3993457-3993729 [Pseudomonas alcaligenes]
ATGGCCGCAACAGCTAAAACCCGATCCGTCACCGCCCACGTCCCTGTAGAGCTGGCCGAACGTGTTGATGAGATTGCCGA
GCGCCTGGAGCGCTCCAAGAACTGGATCGTCAAGCAAGCCCTGTCCGCCTGGATCGACCAGGAAGAAGAGCGCAGCCGCC
TGACCCGCGAGGCTCTGGCCGATGTGGATGCCGGCCGCGTGATCGACCATCAGGCCGTCCAGGCCTGGGCCGACAGCCTG
AGCACAGACACCCCGCTGCCGGTACCGCACTGA