Detailed information of TA system TA038433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 3865731..3866424
• Replicon: chromosome
• Accession: NZ_AP024354
• Organism: Pseudomonas alcaligenes strain KAM426

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: JUH08_RS18600
• Protein ID: WP_203788600.1
• Coordinates: 3866047..3866424 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: JUH08_RS18595
• Protein ID: WP_203788597.1
• Coordinates: 3865731..3866066 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JUH08_RS18555	3860737..3861435	+	699	WP_203788589.1	DNA-binding protein	-
JUH08_RS18560	3861509..3862240	-	732	WP_203788591.1	TonB C-terminal domain-containing protein	-
JUH08_RS18565	3862431..3862979	-	549	WP_203788593.1	hypothetical protein	-
JUH08_RS18570	3863248..3864162	-	915	WP_001736514.1	HD domain-containing protein	-
JUH08_RS18575	3864201..3864536	-	336	WP_203788595.1	DUF3391 domain-containing protein	-
JUH08_RS18580	3864654..3865034	-	381	WP_001054412.1	hypothetical protein	-
JUH08_RS18585	3865031..3865357	-	327	WP_000091614.1	hypothetical protein	-
JUH08_RS18590	3865381..3865716	-	336	WP_000741275.1	hypothetical protein	-
JUH08_RS18595	3865731..3866066	-	336	WP_203788597.1	helix-turn-helix domain-containing protein	Antitoxin
JUH08_RS18600	3866047..3866424	-	378	WP_203788600.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JUH08_RS18605	3866616..3867218	+	603	WP_010465829.1	recombinase family protein	-
JUH08_RS18610	3867202..3869889	+	2688	Protein_3650	Tn3 family transposase	-
JUH08_RS18615	3869939..3870202	-	264	WP_171953601.1	hypothetical protein	-
JUH08_RS18620	3870205..3870480	-	276	WP_116803468.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JUH08_RS18625	3870480..3870752	-	273	WP_061194820.1	ribbon-helix-helix domain-containing protein	-
JUH08_RS18630	3870736..3871320	-	585	WP_061194821.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	sul1 / blaIMP-1	-	3786007..4015023	229016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13792.92 Da
• Isoelectric Point: 9.7862

>T38433 WP_203788600.1 NZ_AP024354:c3866424-3866047 [Pseudomonas alcaligenes]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAM
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKRNH

Nucleotide

• Length: 378 bp

>T38433 NZ_AP024354:c3866424-3866047 [Pseudomonas alcaligenes]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGATG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAAGGAATCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12102.25 Da
• Isoelectric Point: 10.3997

>AT38433 WP_203788597.1 NZ_AP024354:c3866066-3865731 [Pseudomonas alcaligenes]
MQKGIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT38433 NZ_AP024354:c3866066-3865731 [Pseudomonas alcaligenes]
ATGCAAAAAGGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA