Detailed information of TA system TA038402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2655144..2655733
• Replicon: chromosome
• Accession: NZ_AP024342
• Organism: Pseudomonas sp. Eqa60

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7V8EXE5
• Locus tag: KI242_RS12185
• Protein ID: WP_019094202.1
• Coordinates: 2655482..2655733 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KI242_RS12180
• Protein ID: WP_072451388.1
• Coordinates: 2655144..2655485 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI242_RS12155 (PEQA60_24110)	2650488..2651444	-	957	WP_015635066.1	nucleoside-specific channel-forming protein Tsx	-
KI242_RS12160 (PEQA60_24120)	2651707..2652108	+	402	WP_227454131.1	nuclear transport factor 2 family protein	-
KI242_RS12165 (PEQA60_24130)	2652152..2653186	+	1035	WP_015635068.1	selenide, water dikinase SelD	-
KI242_RS12170 (PEQA60_24140)	2653186..2654289	+	1104	WP_210456994.1	tRNA 2-selenouridine(34) synthase MnmH	-
KI242_RS12175 (PEQA60_24150)	2654342..2654683	-	342	WP_053157594.1	DUF3077 domain-containing protein	-
KI242_RS12180 (PEQA60_24160)	2655144..2655485	-	342	WP_072451388.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KI242_RS12185 (PEQA60_24170)	2655482..2655733	-	252	WP_019094202.1	type II toxin-antitoxin system HicA family toxin	Toxin
KI242_RS12190 (PEQA60_24180)	2655821..2656876	-	1056	WP_053157597.1	permease	-
KI242_RS12195 (PEQA60_24190)	2657291..2658067	-	777	WP_019094200.1	IclR family transcriptional regulator	-
KI242_RS12200 (PEQA60_24200)	2658239..2659009	+	771	WP_183000119.1	ABC transporter substrate-binding protein	-
KI242_RS12205 (PEQA60_24210)	2659070..2659726	+	657	WP_011060581.1	amino acid ABC transporter permease	-
KI242_RS12210 (PEQA60_24220)	2659723..2660469	+	747	WP_011060582.1	amino acid ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9239.70 Da
• Isoelectric Point: 10.4707

>T38402 WP_019094202.1 NZ_AP024342:c2655733-2655482 [Pseudomonas sp. Eqa60]
VSKNDKLLAKLLNDRGTFSWQELVTLLKRLGYTPLEGSGSRVKFDNGDPGAMINLHKPHPGNELKAYIRRQVIEQLKSGG
LIP

Nucleotide

• Length: 252 bp

>T38402 NZ_AP024342:c2655733-2655482 [Pseudomonas sp. Eqa60]
GTGTCCAAGAACGATAAGCTTCTCGCCAAGTTACTCAACGACCGAGGGACCTTTTCCTGGCAGGAACTGGTCACTCTCCT
AAAGCGCCTGGGCTACACACCGTTGGAGGGCTCGGGCAGCCGGGTAAAATTCGACAATGGTGATCCAGGCGCAATGATCA
ACCTGCACAAACCGCACCCCGGTAATGAGCTGAAGGCCTACATACGCCGCCAAGTGATCGAGCAACTGAAATCGGGAGGT
CTCATACCGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12438.82 Da
• Isoelectric Point: 4.8281

>AT38402 WP_072451388.1 NZ_AP024342:c2655485-2655144 [Pseudomonas sp. Eqa60]
VNNLLQHKGYYGSIEVSTEDNCLFGKLQFIRALVSYEGQTVAELEAAFKEAVDDYLDTCEQSGTAPEIPCKGSFNVRVGH
DLHLAAAVAATRQNVSLNDLTRQALNEYLQHHG

Nucleotide

• Length: 342 bp

>AT38402 NZ_AP024342:c2655485-2655144 [Pseudomonas sp. Eqa60]
GTGAACAACCTACTGCAGCACAAGGGCTATTACGGCTCTATCGAGGTCAGTACCGAGGACAATTGCCTCTTTGGCAAACT
TCAGTTCATAAGGGCCTTGGTTAGCTATGAGGGGCAGACCGTGGCTGAACTCGAAGCGGCCTTCAAAGAGGCAGTAGACG
ACTATCTGGATACCTGTGAGCAATCGGGTACAGCACCCGAAATCCCTTGCAAAGGCTCGTTCAACGTTCGGGTAGGCCAC
GATCTTCATCTAGCGGCTGCCGTGGCCGCCACCCGGCAGAATGTATCGTTGAACGATCTGACTCGTCAGGCCCTGAATGA
ATACCTGCAACATCACGGCTGA

Structures

Toxin

• AlphaFold DB: A0A7V8EXE5