Detailed information of TA system TA038398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 761875..762471
• Replicon: chromosome
• Accession: NZ_AP024342
• Organism: Pseudomonas sp. Eqa60

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KI242_RS03340
• Protein ID: WP_194237384.1
• Coordinates: 762169..762471 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q4KIY9
• Locus tag: KI242_RS03335
• Protein ID: WP_011059020.1
• Coordinates: 761875..762165 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI242_RS03315 (PEQA60_06560)	758145..760277	-	2133	WP_212805899.1	TonB-dependent copper receptor	-
KI242_RS03320 (PEQA60_06570)	760366..760776	-	411	WP_011059017.1	DUF2946 domain-containing protein	-
KI242_RS03325 (PEQA60_06580)	760832..761326	-	495	WP_244868163.1	copper chaperone PCu(A)C	-
KI242_RS03330 (PEQA60_06590)	761376..761774	-	399	WP_161466589.1	DUF2946 domain-containing protein	-
KI242_RS03335 (PEQA60_06600)	761875..762165	-	291	WP_011059020.1	putative addiction module antidote protein	Antitoxin
KI242_RS03340 (PEQA60_06610)	762169..762471	-	303	WP_194237384.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KI242_RS03345 (PEQA60_06620)	762655..763383	-	729	WP_011059022.1	cobalt-precorrin-6A reductase	-
KI242_RS03350 (PEQA60_06630)	763380..764477	-	1098	WP_053156138.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
KI242_RS03355 (PEQA60_06640)	764571..765785	-	1215	WP_053156141.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
KI242_RS03360 (PEQA60_06650)	765924..767237	+	1314	WP_169893062.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-97)

Antitoxin

• Uncharacterized protein HI1420 (3-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11435.23 Da
• Isoelectric Point: 10.4481

>T38398 WP_194237384.1 NZ_AP024342:c762471-762169 [Pseudomonas sp. Eqa60]
MFVFEQTPEFSRWLLNLKDPQGKTRVLARIRAAQLGRFGDCEPVGEGVHEMRIHRGPGYRVYFSRRGKVIYLLLIGGDKA
TQKRDIQRASQLACDLKSEG

Nucleotide

• Length: 303 bp

>T38398 NZ_AP024342:c762471-762169 [Pseudomonas sp. Eqa60]
ATGTTCGTTTTCGAGCAGACTCCTGAGTTCAGCCGCTGGCTGCTGAACCTGAAGGACCCGCAAGGGAAAACCCGTGTACT
GGCCAGGATCCGCGCCGCACAGCTGGGCCGTTTTGGCGACTGCGAACCTGTGGGCGAAGGCGTCCACGAGATGCGCATCC
ACCGTGGCCCGGGTTACCGGGTGTACTTCTCGCGCCGCGGCAAAGTGATCTACCTGCTGTTGATCGGCGGCGACAAGGCC
ACCCAGAAACGCGATATCCAACGGGCCAGTCAACTGGCCTGCGACCTCAAGAGCGAGGGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10423.94 Da
• Isoelectric Point: 5.0939

>AT38398 WP_011059020.1 NZ_AP024342:c762165-761875 [Pseudomonas sp. Eqa60]
MRTPVTRFDAAEYLKTPEDMVAYLDACFEEDTGDGVLIRAALNDIARARGMTQVARDTGLGRESLYKALGSQGNPEFATI
IKVMKALGLKINVSNA

Nucleotide

• Length: 291 bp

>AT38398 NZ_AP024342:c762165-761875 [Pseudomonas sp. Eqa60]
ATGCGCACCCCAGTGACCCGCTTCGACGCGGCGGAGTACCTCAAGACCCCTGAAGACATGGTCGCCTACCTCGACGCCTG
CTTCGAGGAAGACACCGGCGACGGCGTGCTGATCCGCGCCGCACTCAACGACATTGCCCGTGCCCGCGGCATGACCCAGG
TCGCCCGTGACACCGGCCTGGGCCGCGAAAGCCTGTACAAGGCCCTCGGCAGCCAGGGCAACCCGGAGTTCGCGACTATC
ATCAAGGTGATGAAGGCCCTGGGGTTGAAGATCAACGTCAGCAACGCCTGA

Structures

Antitoxin

• AlphaFold DB: Q4KIY9