Detailed information of TA system TA038371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1144104..1144732
• Replicon: chromosome
• Accession: NZ_AP024329
• Organism: Erwinia rhapontici strain MAFF 311153

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: KJS81_RS05295
• Protein ID: WP_133845760.1
• Coordinates: 1144104..1144322 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: KJS81_RS05300
• Protein ID: WP_133845759.1
• Coordinates: 1144352..1144732 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJS81_RS05265 (ERHA53_10520)	1140073..1140411	+	339	WP_007886947.1	P-II family nitrogen regulator	-
KJS81_RS05270 (ERHA53_10530)	1140431..1141732	+	1302	WP_171148780.1	ammonium transporter AmtB	-
KJS81_RS05275 (ERHA53_10540)	1141827..1142687	-	861	WP_133845762.1	acyl-CoA thioesterase II	-
KJS81_RS05280 (ERHA53_10550)	1142913..1143470	+	558	WP_133845761.1	YbaY family lipoprotein	-
KJS81_RS05285 (ERHA53_10560)	1143500..1143814	-	315	WP_133845785.1	MGMT family protein	-
KJS81_RS05295	1144104..1144322	-	219	WP_133845760.1	HHA domain-containing protein	Toxin
KJS81_RS05300 (ERHA53_10570)	1144352..1144732	-	381	WP_133845759.1	Hha toxicity modulator TomB	Antitoxin
KJS81_RS05305 (ERHA53_10580)	1144880..1145233	-	354	WP_159337511.1	hypothetical protein	-
KJS81_RS24215	1145520..1145645	+	126	WP_259151068.1	hypothetical protein	-
KJS81_RS05310 (ERHA53_10590)	1145645..1146334	+	690	WP_159337510.1	ATP-binding cassette domain-containing protein	-
KJS81_RS05315 (ERHA53_10600)	1146331..1147194	+	864	WP_133845756.1	metal ABC transporter permease	-
KJS81_RS05320 (ERHA53_10610)	1147197..1148075	+	879	WP_133845755.1	metal ABC transporter substrate-binding protein	-
KJS81_RS05325 (ERHA53_10620)	1148190..1148333	-	144	WP_012442195.1	type B 50S ribosomal protein L36	-
KJS81_RS05330 (ERHA53_10630)	1148343..1148603	-	261	WP_133845754.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8655.11 Da
• Isoelectric Point: 8.9007

>T38371 WP_133845760.1 NZ_AP024329:c1144322-1144104 [Erwinia rhapontici]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAIFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 219 bp

>T38371 NZ_AP024329:c1144322-1144104 [Erwinia rhapontici]
ATGACAGACAAACTTTTAACGAAAACTGATTATCTTATGCGCCTCAGACGTTGTCGTTCTATCGACACGCTTGAGCGCGT
GATTGAAAAGAACAAGTACGAATTATCTGATGACGAATTAGCCATATTTTATTCTGCTGCCGATCACCGTTTGGCGGAAC
TCACAATGAATAAGCTTTATGACAAGATACCGGTAGCGGTCTGGAAGTTTGTGCGTTAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14562.10 Da
• Isoelectric Point: 4.5911

>AT38371 WP_133845759.1 NZ_AP024329:c1144732-1144352 [Erwinia rhapontici]
MDEYSPRRHDIAQLKFLSESLYDESLATLGDSHHGWVNDPTSAANLQLNDLIEHIAAFTMNYKIKHIEDSDLITQVDEYL
DDTFMLFSNYGINAQDLQRWQKSAKRLFNIFAEECATSRTQASHSF

Nucleotide

• Length: 381 bp

>AT38371 NZ_AP024329:c1144732-1144352 [Erwinia rhapontici]
ATGGACGAATACTCGCCGAGAAGGCATGATATTGCCCAGCTTAAATTCTTGAGTGAAAGCCTGTATGATGAAAGCCTTGC
AACGTTAGGCGACAGCCATCACGGCTGGGTTAATGATCCTACCTCTGCTGCCAATCTACAGCTTAACGATCTGATTGAGC
ATATTGCGGCATTCACGATGAATTACAAAATTAAGCACATTGAGGATAGTGATCTGATAACTCAGGTTGATGAATATCTT
GATGACACCTTCATGTTGTTCAGTAATTACGGCATTAATGCTCAGGACTTACAGCGCTGGCAGAAATCAGCAAAGAGGCT
GTTCAATATTTTTGCAGAAGAATGTGCAACAAGTCGGACCCAGGCCAGCCATTCCTTTTAG