Detailed information of TA system TA038362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2071681..2072210
• Replicon: chromosome
• Accession: NZ_AP024318
• Organism: Staphylococcus aureus strain S36

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K5606_RS10170
• Protein ID: WP_000621175.1
• Coordinates: 2071681..2072043 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: K5606_RS10175
• Protein ID: WP_000948331.1
• Coordinates: 2072040..2072210 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5606_RS10150 (2068659)	2068659..2069429	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
K5606_RS10155 (2069404)	2069404..2069883	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
K5606_RS10160 (2069885)	2069885..2070211	-	327	WP_001052491.1	anti-sigma factor antagonist	-
K5606_RS10165 (2070330)	2070330..2071331	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
K5606_RS10170 (2071681)	2071681..2072043	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K5606_RS10175 (2072040)	2072040..2072210	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
K5606_RS10180 (2072295)	2072295..2073443	-	1149	WP_001281139.1	alanine racemase	-
K5606_RS10185 (2073509)	2073509..2073868	-	360	WP_000581197.1	holo-ACP synthase	-
K5606_RS10190 (2073872)	2073872..2074363	-	492	WP_001286980.1	PH domain-containing protein	-
K5606_RS10195 (2074356)	2074356..2075933	-	1578	WP_001294650.1	PH domain-containing protein	-
K5606_RS10200 (2075926)	2075926..2076405	-	480	WP_001287081.1	hypothetical protein	-
K5606_RS10205 (2076614)	2076614..2077174	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T38362 WP_000621175.1 NZ_AP024318:c2072043-2071681 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T38362 NZ_AP024318:c2072043-2071681 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT38362 WP_000948331.1 NZ_AP024318:c2072210-2072040 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT38362 NZ_AP024318:c2072210-2072040 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1