Detailed information of TA system TA038350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2103893..2104422
• Replicon: chromosome
• Accession: NZ_AP024315
• Organism: Staphylococcus aureus strain BDH17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K5602_RS10305
• Protein ID: WP_000621175.1
• Coordinates: 2103893..2104255 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: K5602_RS10310
• Protein ID: WP_000948331.1
• Coordinates: 2104252..2104422 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5602_RS10285 (2100871)	2100871..2101641	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
K5602_RS10290 (2101616)	2101616..2102095	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
K5602_RS10295 (2102097)	2102097..2102423	-	327	WP_001052491.1	anti-sigma factor antagonist	-
K5602_RS10300 (2102542)	2102542..2103543	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
K5602_RS10305 (2103893)	2103893..2104255	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K5602_RS10310 (2104252)	2104252..2104422	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
K5602_RS10315 (2104507)	2104507..2105655	-	1149	WP_001281139.1	alanine racemase	-
K5602_RS10320 (2105721)	2105721..2106080	-	360	WP_000581197.1	holo-ACP synthase	-
K5602_RS10325 (2106084)	2106084..2106575	-	492	WP_001286980.1	PH domain-containing protein	-
K5602_RS10330 (2106568)	2106568..2108145	-	1578	WP_001294650.1	PH domain-containing protein	-
K5602_RS10335 (2108138)	2108138..2108617	-	480	WP_001287081.1	hypothetical protein	-
K5602_RS10340 (2108826)	2108826..2109386	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T38350 WP_000621175.1 NZ_AP024315:c2104255-2103893 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T38350 NZ_AP024315:c2104255-2103893 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT38350 WP_000948331.1 NZ_AP024315:c2104422-2104252 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT38350 NZ_AP024315:c2104422-2104252 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1