Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2184466..2184980 Replicon chromosome
Accession NZ_AP024313
Organism Staphylococcus aureus strain N1195

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag K5605_RS10815 Protein ID WP_000581792.1
Coordinates 2184466..2184675 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag K5605_RS10820 Protein ID WP_000138932.1
Coordinates 2184687..2184980 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5605_RS10795 (2179977) 2179977..2181332 - 1356 WP_221046795.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
K5605_RS10800 (2181347) 2181347..2182417 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
K5605_RS10805 (2182735) 2182735..2183937 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
K5605_RS10810 (2184201) 2184201..2184338 - 138 WP_000828354.1 Lmo0850 family protein -
K5605_RS10815 (2184466) 2184466..2184675 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
K5605_RS10820 (2184687) 2184687..2184980 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
K5605_RS10825 (2185146) 2185146..2186630 + 1485 WP_000571550.1 cardiolipin synthase -
K5605_RS10830 (2186658) 2186658..2187305 + 648 WP_001187614.1 HD domain-containing protein -
K5605_RS14200 (2187862) 2187862..2187924 + 63 Protein_2107 hypothetical protein -
K5605_RS10840 (2187902) 2187902..2189217 - 1316 Protein_2108 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T38339 WP_000581792.1 NZ_AP024313:c2184675-2184466 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T38339 NZ_AP024313:c2184675-2184466 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT38339 WP_000138932.1 NZ_AP024313:c2184980-2184687 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT38339 NZ_AP024313:c2184980-2184687 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References