Detailed information of TA system TA038338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2164729..2165258
• Replicon: chromosome
• Accession: NZ_AP024313
• Organism: Staphylococcus aureus strain N1195

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K5605_RS10725
• Protein ID: WP_000621175.1
• Coordinates: 2164729..2165091 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: K5605_RS10730
• Protein ID: WP_000948331.1
• Coordinates: 2165088..2165258 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5605_RS10705 (2161707)	2161707..2162477	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
K5605_RS10710 (2162452)	2162452..2162931	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
K5605_RS10715 (2162933)	2162933..2163259	-	327	WP_001052491.1	anti-sigma factor antagonist	-
K5605_RS10720 (2163378)	2163378..2164379	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
K5605_RS10725 (2164729)	2164729..2165091	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K5605_RS10730 (2165088)	2165088..2165258	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
K5605_RS10735 (2165343)	2165343..2166491	-	1149	WP_001281139.1	alanine racemase	-
K5605_RS10740 (2166557)	2166557..2166916	-	360	WP_000581197.1	holo-ACP synthase	-
K5605_RS10745 (2166920)	2166920..2167411	-	492	WP_001286980.1	PH domain-containing protein	-
K5605_RS10750 (2167404)	2167404..2168981	-	1578	WP_001294650.1	PH domain-containing protein	-
K5605_RS10755 (2168974)	2168974..2169453	-	480	WP_001287081.1	hypothetical protein	-
K5605_RS10760 (2169662)	2169662..2170222	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T38338 WP_000621175.1 NZ_AP024313:c2165091-2164729 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T38338 NZ_AP024313:c2165091-2164729 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT38338 WP_000948331.1 NZ_AP024313:c2165258-2165088 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT38338 NZ_AP024313:c2165258-2165088 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1