Detailed information of TA system TA038326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2168707..2169236
• Replicon: chromosome
• Accession: NZ_AP024311
• Organism: Staphylococcus aureus strain THI2018-120

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ST1232TP_RS10750
• Protein ID: WP_000621175.1
• Coordinates: 2168707..2169069 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: ST1232TP_RS10755
• Protein ID: WP_000948331.1
• Coordinates: 2169066..2169236 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ST1232TP_RS10730 (2165685)	2165685..2166455	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
ST1232TP_RS10735 (2166430)	2166430..2166909	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
ST1232TP_RS10740 (2166911)	2166911..2167237	-	327	WP_001052491.1	anti-sigma factor antagonist	-
ST1232TP_RS10745 (2167356)	2167356..2168357	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ST1232TP_RS10750 (2168707)	2168707..2169069	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ST1232TP_RS10755 (2169066)	2169066..2169236	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ST1232TP_RS10760 (2169321)	2169321..2170469	-	1149	WP_001281139.1	alanine racemase	-
ST1232TP_RS10765 (2170535)	2170535..2170894	-	360	WP_000581197.1	holo-ACP synthase	-
ST1232TP_RS10770 (2170898)	2170898..2171389	-	492	WP_001286980.1	PH domain-containing protein	-
ST1232TP_RS10775 (2171382)	2171382..2172959	-	1578	WP_001294650.1	PH domain-containing protein	-
ST1232TP_RS10780 (2172952)	2172952..2173431	-	480	WP_001287081.1	hypothetical protein	-
ST1232TP_RS10785 (2173640)	2173640..2174200	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T38326 WP_000621175.1 NZ_AP024311:c2169069-2168707 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T38326 NZ_AP024311:c2169069-2168707 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT38326 WP_000948331.1 NZ_AP024311:c2169236-2169066 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT38326 NZ_AP024311:c2169236-2169066 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1