Detailed information of TA system TA038319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 4168497..4169161
• Replicon: chromosome
• Accession: NZ_AP024310
• Organism: Mycobacterium heckeshornense strain JMUB5695

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JUH53_RS19980
• Protein ID: WP_203801313.1
• Coordinates: 4168497..4168904 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2G8BJ38
• Locus tag: JUH53_RS19985
• Protein ID: WP_048892808.1
• Coordinates: 4168901..4169161 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JUH53_RS19970	4166044..4166316	-	273	Protein_3941	IS3 family transposase	-
JUH53_RS19975	4166549..4167175	-	627	WP_048892804.1	hypothetical protein	-
JUH53_RS19980	4168497..4168904	-	408	WP_203801313.1	type II toxin-antitoxin system VapC family toxin	Toxin
JUH53_RS19985	4168901..4169161	-	261	WP_048892808.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JUH53_RS19990	4169611..4171917	-	2307	WP_203801315.1	GH92 family glycosyl hydrolase	-
JUH53_RS19995	4172214..4172642	-	429	WP_203801317.1	DUF86 domain-containing protein	-
JUH53_RS20000	4172632..4173015	-	384	WP_203801319.1	nucleotidyltransferase domain-containing protein	-
JUH53_RS20005	4173306..4174070	+	765	WP_203801321.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4166029..4166193	164

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14326.32 Da
• Isoelectric Point: 4.2062

>T38319 WP_203801313.1 NZ_AP024310:c4168904-4168497 [Mycobacterium heckeshornense]
MSETPPAGLLDTSVFIASETGRQLEESLIPAEVATTVITVAELTVGVLAASTSDSRAQRLATLESVADMETLPVDEEAAR
MWARLRIHLAETGRRVRINDLWIAAIAASRALPVVTQDDDFAALDGAAGLTIIKV

Nucleotide

• Length: 408 bp

>T38319 NZ_AP024310:c4168904-4168497 [Mycobacterium heckeshornense]
ATGAGCGAGACGCCGCCGGCGGGGCTGCTTGACACATCGGTGTTCATCGCCTCCGAAACGGGCCGTCAACTCGAGGAGTC
GCTGATCCCTGCCGAGGTCGCGACTACTGTGATCACTGTTGCCGAACTGACCGTCGGCGTGCTGGCCGCAAGCACTTCCG
ATAGCCGGGCGCAGCGACTCGCGACCCTCGAATCCGTTGCGGATATGGAAACGCTGCCGGTCGATGAGGAAGCGGCGCGC
ATGTGGGCGCGGCTACGCATCCACCTGGCCGAGACTGGCCGTCGAGTCCGTATCAACGACCTGTGGATCGCAGCCATCGC
TGCTTCGCGAGCGCTGCCCGTCGTCACCCAGGACGACGACTTTGCCGCCCTGGACGGCGCGGCCGGTCTCACGATCATCA
AAGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9323.63 Da
• Isoelectric Point: 11.1777

>AT38319 WP_048892808.1 NZ_AP024310:c4169161-4168901 [Mycobacterium heckeshornense]
MSDVASRDLRNDTAGVLRRVQAGEDITITVKGRPVAMLTAAGPPRRRWLAKTEFLARLRRAQADPGLRDDLGLLAGDTTD
DLGPAR

Nucleotide

• Length: 261 bp

>AT38319 NZ_AP024310:c4169161-4168901 [Mycobacterium heckeshornense]
ATGTCCGATGTGGCTTCCCGTGACTTGCGCAACGATACGGCGGGAGTGCTTCGCCGCGTCCAGGCCGGAGAAGACATCAC
CATCACGGTCAAAGGCCGCCCCGTCGCAATGCTCACCGCGGCCGGGCCTCCGCGTCGGCGCTGGTTGGCCAAAACCGAGT
TTCTGGCACGGTTGCGCCGTGCGCAAGCCGATCCAGGTTTGCGCGACGACCTTGGGTTGCTCGCCGGCGACACCACCGAC
GATCTCGGTCCTGCCCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A2G8BJ38