Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4842932..4843548 Replicon chromosome
Accession NZ_AP024281
Organism Enterobacter asburiae strain Ent261

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag R1N_RS23565 Protein ID WP_080277799.1
Coordinates 4842932..4843303 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag R1N_RS23570 Protein ID WP_033146961.1
Coordinates 4843306..4843548 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R1N_RS23550 (R1N_46200) 4840432..4841334 + 903 WP_010436855.1 formate dehydrogenase subunit beta -
R1N_RS23555 (R1N_46210) 4841331..4841966 + 636 WP_010436852.1 formate dehydrogenase cytochrome b556 subunit -
R1N_RS23560 (R1N_46220) 4841963..4842892 + 930 WP_023309751.1 formate dehydrogenase accessory protein FdhE -
R1N_RS23565 (R1N_46230) 4842932..4843303 - 372 WP_080277799.1 type II toxin-antitoxin system VapC family toxin Toxin
R1N_RS23570 (R1N_46240) 4843306..4843548 - 243 WP_033146961.1 CopG family transcriptional regulator Antitoxin
R1N_RS23575 (R1N_46250) 4843748..4844668 + 921 WP_033146962.1 alpha/beta hydrolase -
R1N_RS23580 (R1N_46260) 4844677..4845618 - 942 WP_033146963.1 fatty acid biosynthesis protein FabY -
R1N_RS23585 (R1N_46270) 4845663..4846100 - 438 WP_033146964.1 D-aminoacyl-tRNA deacylase -
R1N_RS23590 (R1N_46280) 4846097..4846978 - 882 WP_033146965.1 virulence factor BrkB family protein -
R1N_RS23595 (R1N_46290) 4846972..4847571 - 600 WP_033146966.1 glucose-1-phosphatase -
R1N_RS23600 (R1N_46300) 4847660..4848130 - 471 WP_033146967.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-109)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13687.90 Da        Isoelectric Point: 6.9789

>T38307 WP_080277799.1 NZ_AP024281:c4843303-4842932 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRSCPLISRNTKDFLGITGVVSPYQL

Download         Length: 372 bp

>T38307 NZ_AP024281:c4843303-4842932 [Enterobacter asburiae]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCTCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTTTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAGTTGCCCGTTAATCTCACGCAA
TACGAAAGACTTTTTGGGCATCACCGGGGTCGTTTCACCTTACCAGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9469.67 Da        Isoelectric Point: 4.2917

>AT38307 WP_033146961.1 NZ_AP024281:c4843548-4843306 [Enterobacter asburiae]
MILSADLDMGRILIDLSEDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Download         Length: 243 bp

>AT38307 NZ_AP024281:c4843548-4843306 [Enterobacter asburiae]
ATGATTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGAAGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References