Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3130986..3131576 Replicon chromosome
Accession NZ_AP024281
Organism Enterobacter asburiae strain Ent261

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag R1N_RS15270 Protein ID WP_033145927.1
Coordinates 3130986..3131318 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag R1N_RS15275 Protein ID WP_033145928.1
Coordinates 3131319..3131576 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R1N_RS15245 (R1N_30050) 3126147..3126950 - 804 WP_003036327.1 TIGR02391 family protein -
R1N_RS15250 (R1N_30060) 3126972..3127454 - 483 WP_003036324.1 hypothetical protein -
R1N_RS15255 (R1N_30070) 3127530..3128468 - 939 WP_003036321.1 hypothetical protein -
R1N_RS15260 (R1N_30090) 3129304..3130665 + 1362 WP_003036316.1 HEPN/Toprim-associated domain-containing protein -
R1N_RS15265 3130835..3131029 + 195 WP_080277779.1 hypothetical protein -
R1N_RS15270 (R1N_30100) 3130986..3131318 - 333 WP_033145927.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
R1N_RS15275 (R1N_30110) 3131319..3131576 - 258 WP_033145928.1 antitoxin Antitoxin
R1N_RS15280 (R1N_30120) 3131868..3132257 - 390 WP_033145929.1 RidA family protein -
R1N_RS15285 (R1N_30130) 3132399..3133319 + 921 WP_033145930.1 LysR family transcriptional regulator -
R1N_RS15290 (R1N_30140) 3133395..3133697 - 303 WP_029741460.1 XRE family transcriptional regulator -
R1N_RS15295 (R1N_30150) 3133702..3134055 - 354 WP_033145931.1 type II toxin-antitoxin system RelE/ParE family toxin -
R1N_RS15300 (R1N_30160) 3134233..3135126 - 894 WP_033145932.1 LysR substrate-binding domain-containing protein -
R1N_RS15305 (R1N_30170) 3135297..3135905 + 609 WP_080277780.1 glutathione S-transferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11828.65 Da        Isoelectric Point: 9.4051

>T38303 WP_033145927.1 NZ_AP024281:c3131318-3130986 [Enterobacter asburiae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEGAGTTTTGVIRCDQPRTI
DMSARNGMRLERIPDAVINEVLARLDAILN

Download         Length: 333 bp

>T38303 NZ_AP024281:c3131318-3130986 [Enterobacter asburiae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTAGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACCGTTTCACTGGAAGGGGCGGGAACAACAACAACGGGCGTTATTCGCTGCGATCAGCCCAGAACCATT
GATATGTCTGCCCGGAACGGCATGCGGCTGGAACGTATACCTGACGCGGTTATAAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTGAATTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9464.92 Da        Isoelectric Point: 4.4737

>AT38303 WP_033145928.1 NZ_AP024281:c3131576-3131319 [Enterobacter asburiae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEEREWIDAPAV
GREIL

Download         Length: 258 bp

>AT38303 NZ_AP024281:c3131576-3131319 [Enterobacter asburiae]
ATGTATACAACTCGTCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAACT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAGCGGCCTCACTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCTGAAATGAGCGAAGAGGAGCGGGAATGGATCGATGCGCCTGCGGTG
GGCAGGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References