Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | kacAT/DUF1778(antitoxin) |
| Location | 1777983..1778780 | Replicon | chromosome |
| Accession | NZ_AP024281 | ||
| Organism | Enterobacter asburiae strain Ent261 | ||
Toxin (Protein)
| Gene name | KacT | Uniprot ID | - |
| Locus tag | R1N_RS08625 | Protein ID | WP_033145162.1 |
| Coordinates | 1778259..1778780 (+) | Length | 174 a.a. |
Antitoxin (Protein)
| Gene name | KacA | Uniprot ID | W7PG43 |
| Locus tag | R1N_RS08620 | Protein ID | WP_008499829.1 |
| Coordinates | 1777983..1778252 (+) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| R1N_RS08590 (R1N_16850) | 1773610..1774053 | - | 444 | WP_032658095.1 | helix-turn-helix domain-containing protein | - |
| R1N_RS08595 (R1N_16860) | 1774206..1775447 | + | 1242 | WP_033145159.1 | bifunctional glucose-1-phosphatase/inositol phosphatase | - |
| R1N_RS08600 (R1N_16870) | 1775481..1775708 | - | 228 | WP_033145160.1 | YccJ family protein | - |
| R1N_RS08605 (R1N_16880) | 1775729..1776325 | - | 597 | WP_023311076.1 | NAD(P)H:quinone oxidoreductase | - |
| R1N_RS08610 (R1N_16890) | 1776716..1776886 | + | 171 | WP_023311077.1 | general stress protein | - |
| R1N_RS08615 (R1N_16900) | 1777004..1777921 | + | 918 | WP_033145161.1 | DMT family transporter | - |
| R1N_RS08620 (R1N_16910) | 1777983..1778252 | + | 270 | WP_008499829.1 | DUF1778 domain-containing protein | Antitoxin |
| R1N_RS08625 (R1N_16920) | 1778259..1778780 | + | 522 | WP_033145162.1 | GNAT family N-acetyltransferase | Toxin |
| R1N_RS08630 (R1N_16930) | 1778854..1780176 | - | 1323 | WP_033145163.1 | pyrimidine utilization transport protein G | - |
| R1N_RS08635 (R1N_16940) | 1780198..1780692 | - | 495 | WP_032658088.1 | pyrimidine utilization flavin reductase protein F | - |
| R1N_RS08640 (R1N_16950) | 1780702..1781292 | - | 591 | WP_033145164.1 | malonic semialdehyde reductase | - |
| R1N_RS08645 (R1N_16960) | 1781302..1782102 | - | 801 | WP_033145165.1 | pyrimidine utilization protein D | - |
| R1N_RS08650 (R1N_16970) | 1782110..1782496 | - | 387 | WP_033145166.1 | pyrimidine utilization protein C | - |
| R1N_RS08655 (R1N_16980) | 1782508..1783197 | - | 690 | WP_033145167.1 | pyrimidine utilization protein B | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 174 a.a. Molecular weight: 19768.56 Da Isoelectric Point: 6.9697
>T38294 WP_033145162.1 NZ_AP024281:1778259-1778780 [Enterobacter asburiae]
VENLTIEMLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQR
RIPYINVPSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTAENSHSLF
FPTKSIERLFEQE
VENLTIEMLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQR
RIPYINVPSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTAENSHSLF
FPTKSIERLFEQE
Download Length: 522 bp
>T38294 NZ_AP024281:1778259-1778780 [Enterobacter asburiae]
GTGGAGAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTATGACTTCGAGGATTTTGATTGTGGCGAACCCTCGCT
AAATGCCTTCCTTGCTGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATC
GTCCGCGAGTCCTGGGTTATTACACTTTATCGGGAAGCTGTTTTGAAAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGC
CGAATCCCTTATATCAACGTACCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGG
AACAACGCTCGTTGCGCATGCTATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATG
CCCTTAACGAGCGGGCAAAACGATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGCTGAAAACAGCCATTCTTTGTTC
TTCCCTACAAAATCTATCGAACGGCTGTTTGAGCAAGAGTGA
GTGGAGAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTATGACTTCGAGGATTTTGATTGTGGCGAACCCTCGCT
AAATGCCTTCCTTGCTGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATC
GTCCGCGAGTCCTGGGTTATTACACTTTATCGGGAAGCTGTTTTGAAAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGC
CGAATCCCTTATATCAACGTACCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGG
AACAACGCTCGTTGCGCATGCTATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATG
CCCTTAACGAGCGGGCAAAACGATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGCTGAAAACAGCCATTCTTTGTTC
TTCCCTACAAAATCTATCGAACGGCTGTTTGAGCAAGAGTGA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10262.72 Da Isoelectric Point: 5.8836
>AT38294 WP_008499829.1 NZ_AP024281:1777983-1778252 [Enterobacter asburiae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME
Download Length: 270 bp
>AT38294 NZ_AP024281:1777983-1778252 [Enterobacter asburiae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGAATGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGAATGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|