Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1061820..1062440 Replicon chromosome
Accession NZ_AP024281
Organism Enterobacter asburiae strain Ent261

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag R1N_RS05040 Protein ID WP_008499287.1
Coordinates 1061820..1062038 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag R1N_RS05045 Protein ID WP_008499288.1
Coordinates 1062066..1062440 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R1N_RS05010 (R1N_09760) 1057834..1058094 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
R1N_RS05015 1058097..1058237 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
R1N_RS05020 (R1N_09770) 1058234..1058944 - 711 WP_033144788.1 GNAT family protein -
R1N_RS05025 (R1N_09780) 1059046..1060506 + 1461 WP_033144789.1 PLP-dependent aminotransferase family protein -
R1N_RS05030 (R1N_09790) 1060478..1060945 - 468 WP_023310598.1 YlaC family protein -
R1N_RS05035 (R1N_09800) 1061063..1061614 - 552 WP_033144790.1 maltose O-acetyltransferase -
R1N_RS05040 (R1N_09810) 1061820..1062038 - 219 WP_008499287.1 HHA domain-containing protein Toxin
R1N_RS05045 (R1N_09820) 1062066..1062440 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
R1N_RS05050 (R1N_09830) 1062847..1063827 - 981 WP_000019402.1 IS5-like element IS5 family transposase -
R1N_RS05055 (R1N_09840) 1064151..1067297 - 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit AcrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1062847..1063827 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T38293 WP_008499287.1 NZ_AP024281:c1062038-1061820 [Enterobacter asburiae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T38293 NZ_AP024281:c1062038-1061820 [Enterobacter asburiae]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGGCGTTGTCAGTCAATTGACACGCTTGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAACTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT38293 WP_008499288.1 NZ_AP024281:c1062440-1062066 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT38293 NZ_AP024281:c1062440-1062066 [Enterobacter asburiae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACATCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCGCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References