Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 595975..596551 Replicon chromosome
Accession NZ_AP024281
Organism Enterobacter asburiae strain Ent261

Toxin (Protein)


Gene name shpA Uniprot ID A0A4R0G892
Locus tag R1N_RS02820 Protein ID WP_010427219.1
Coordinates 595975..596262 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A411GC49
Locus tag R1N_RS02825 Protein ID WP_033144548.1
Coordinates 596249..596551 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R1N_RS02795 (R1N_05470) 591091..591786 + 696 WP_033144544.1 winged helix-turn-helix domain-containing protein -
R1N_RS02800 (R1N_05480) 591936..592406 + 471 WP_033144545.1 MarR family transcriptional regulator -
R1N_RS02805 (R1N_05490) 592403..593470 + 1068 WP_101744430.1 HlyD family secretion protein -
R1N_RS02810 (R1N_05500) 593460..594536 + 1077 WP_023310256.1 DUF2955 domain-containing protein -
R1N_RS02815 (R1N_05510) 594533..595804 - 1272 WP_033144547.1 DUF445 domain-containing protein -
R1N_RS02820 (R1N_05520) 595975..596262 + 288 WP_010427219.1 BrnT family toxin Toxin
R1N_RS02825 (R1N_05530) 596249..596551 + 303 WP_033144548.1 BrnA antitoxin family protein Antitoxin
R1N_RS02830 (R1N_05540) 596582..597220 - 639 WP_033144549.1 LysE family translocator -
R1N_RS02835 (R1N_05550) 597268..598011 - 744 WP_033146481.1 AraC family transcriptional regulator -
R1N_RS02840 (R1N_05560) 598163..599533 + 1371 WP_033144550.1 NAD-dependent succinate-semialdehyde dehydrogenase -
R1N_RS02845 (R1N_05570) 599580..599900 - 321 WP_033144551.1 hypothetical protein -
R1N_RS02850 (R1N_05580) 599900..600445 - 546 WP_023310269.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11174.64 Da        Isoelectric Point: 6.7609

>T38292 WP_010427219.1 NZ_AP024281:595975-596262 [Enterobacter asburiae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T38292 NZ_AP024281:595975-596262 [Enterobacter asburiae]
ATGCCAACGGAGTTTGAATGGGATACCAACAAAGCGAAAAGCAATCTCATTAAGCATGGGATCCGCTTTGAAGAAGCTGT
TCTGGTGTTTGACGATCCTTACCATTTATCTTTGCAGGATCGTCATGAAAATGGAGAGTTCCGTTGGCAGACCATTGGCC
TTGTACATGGTCTAATTGTCATTATGGTCGCACACACGGTACGTTTTGAAAGTGGTGACGAAGTCATTCGAATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAGCCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11395.04 Da        Isoelectric Point: 10.2483

>AT38292 WP_033144548.1 NZ_AP024281:596249-596551 [Enterobacter asburiae]
MSMVKHKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT38292 NZ_AP024281:596249-596551 [Enterobacter asburiae]
ATGAGCATGGTTAAACATAAACGCGGTAGTGCGCCTGAGATGAGTGCTCGCCGTGAGGCTGAGCTCAAAGCACTAGCCGA
AAAATCGGAAGCAGAGATTGATTACAGCGATATCCCTGCCACGGGAGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTCAAAACTCAGGCGTCTGTACGTATCGACGCTGACGTGATGGAGTGGCTTAAACGGCCTGGAAAAGGT
TACCAGACGAGGCTCAATGCCATTTTGCGTGAAGCTATGCTGCGCGATCAAAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0G892

Antitoxin

Source ID Structure
AlphaFold DB A0A411GC49

References