Detailed information of TA system TA038290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 256907..257499
• Replicon: chromosome
• Accession: NZ_AP024281
• Organism: Enterobacter asburiae strain Ent261

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: R1N_RS01180
• Protein ID: WP_033144376.1
• Coordinates: 257197..257499 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A808IFF3
• Locus tag: R1N_RS01175
• Protein ID: WP_033144375.1
• Coordinates: 256907..257194 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R1N_RS01170 (R1N_02280)	255279..256910	+	1632	WP_033144374.1	Na/Pi cotransporter family protein	-
R1N_RS01175 (R1N_02290)	256907..257194	-	288	WP_033144375.1	putative addiction module antidote protein	Antitoxin
R1N_RS01180 (R1N_02300)	257197..257499	-	303	WP_033144376.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
R1N_RS01185 (R1N_02310)	257697..258569	+	873	WP_033144377.1	23S rRNA pseudouridine(2604) synthase RluF	-
R1N_RS01190 (R1N_02320)	258570..258842	-	273	WP_023309994.1	DUF3811 domain-containing protein	-
R1N_RS01195 (R1N_02330)	258893..259837	-	945	WP_033144378.1	ketopantoate/pantoate/pantothenate transporter PanS	-
R1N_RS01200 (R1N_02340)	259931..261280	-	1350	WP_033144379.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-89)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11284.02 Da
• Isoelectric Point: 9.9325

>T38290 WP_033144376.1 NZ_AP024281:c257499-257197 [Enterobacter asburiae]
MKEIVQTESFQRWEQNLKDRRAKTIIASRLFRLANGLAGDIKPVGEGISELRIHYGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNPNGRSE

Nucleotide

• Length: 303 bp

>T38290 NZ_AP024281:c257499-257197 [Enterobacter asburiae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCAACGCTGGGAGCAAAACTTAAAAGATCGGCGAGCGAAAACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGTTTAGCGGGCGACATTAAACCCGTGGGCGAAGGTATAAGTGAGCTGAGGATCC
ACTATGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATCCCAATGGCAGGAGTGAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10111.59 Da
• Isoelectric Point: 6.4596

>AT38290 WP_033144375.1 NZ_AP024281:c257194-256907 [Enterobacter asburiae]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKHAGD

Nucleotide

• Length: 288 bp

>AT38290 NZ_AP024281:c257194-256907 [Enterobacter asburiae]
ATGCATAAATTAACACCCTACGATCCAGCCAATGCGCTGGTGGATGACGAGGAAATCGCCGTGTTTATGGCTGATGCCTT
AGAAACGGGCGATTCAGCGTATATTGCTAAAGCTCTGGGCGTCATCGCGCGAGCGAAAGGAATGTCGACCATTTCACAGC
AAACTGGCCTATCACGAGAGCAACTGTATCGATCGTTCAGTGATAAAGGAAACCCAACGCTTAAAACCACTCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTAACAATCAAACACGCTGGAGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A808IFF3