Detailed information of TA system TA038274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2643692..2644309
• Replicon: chromosome
• Accession: NZ_AP024269
• Organism: Kaistia sp. 32K

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: K32_RS12070
• Protein ID: WP_201404472.1
• Coordinates: 2643692..2643880 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: K32_RS12075
• Protein ID: WP_201404236.1
• Coordinates: 2643905..2644309 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K32_RS12035	2638855..2639370	-	516	WP_201404229.1	DUF4142 domain-containing protein	-
K32_RS12040	2639674..2639913	+	240	WP_201404230.1	hypothetical protein	-
K32_RS12045	2640008..2640319	-	312	WP_201404231.1	hypothetical protein	-
K32_RS12050	2640589..2641608	+	1020	WP_201404232.1	PAS domain-containing protein	-
K32_RS12055	2641583..2641960	+	378	WP_201404233.1	response regulator	-
K32_RS12060	2642202..2643257	-	1056	WP_201404234.1	hypothetical protein	-
K32_RS12065	2643373..2643549	-	177	WP_201404235.1	hypothetical protein	-
K32_RS12070	2643692..2643880	+	189	WP_201404472.1	type II toxin-antitoxin system HicA family toxin	Toxin
K32_RS12075	2643905..2644309	+	405	WP_201404236.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K32_RS12080	2644323..2644610	-	288	WP_201404237.1	hypothetical protein	-
K32_RS12085	2644607..2645728	-	1122	WP_201404238.1	PcfJ domain-containing protein	-
K32_RS12090	2645835..2646407	-	573	WP_201404239.1	hypothetical protein	-
K32_RS12095	2646404..2646991	-	588	WP_201404240.1	hypothetical protein	-
K32_RS12100	2647174..2647443	-	270	WP_201404241.1	hypothetical protein	-
K32_RS12105	2647463..2648053	-	591	WP_201404242.1	hypothetical protein	-
K32_RS12110	2648319..2648714	+	396	WP_201404243.1	helix-turn-helix domain-containing protein	-
K32_RS12115	2648711..2648941	-	231	WP_201404244.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2585183..2653765	68582

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-61)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7118.27 Da
• Isoelectric Point: 10.9610

>T38274 WP_201404472.1 NZ_AP024269:2643692-2643880 [Kaistia sp. 32K]
IVSDSREIIRKLQKDGWFEVGVTGSHHHFKHPTKPGKVTVPHPKKDLPIKKVQSIEKQAGWR

Nucleotide

• Length: 189 bp

>T38274 NZ_AP024269:2643692-2643880 [Kaistia sp. 32K]
ATCGTGAGCGACAGCCGGGAAATCATCCGGAAGCTTCAAAAGGACGGGTGGTTCGAGGTCGGCGTGACCGGATCGCACCA
CCACTTCAAGCACCCGACCAAGCCCGGAAAGGTCACGGTCCCGCACCCAAAGAAGGACCTGCCCATCAAGAAGGTGCAGT
CGATCGAGAAACAGGCGGGCTGGCGCTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14042.84 Da
• Isoelectric Point: 4.2783

>AT38274 WP_201404236.1 NZ_AP024269:2643905-2644309 [Kaistia sp. 32K]
MRYVAFIHKDPGSVYGVSFPDFPGCISAGDTADEAVEQAAEALSGHVAMMAADGDAIPAPRSLEEILADPAIEEEREGAS
FAFVPLIREQGAKVPVNISLDAGLLESIDEAARKRGITRSAFLASAARKEIVAA

Nucleotide

• Length: 405 bp

>AT38274 NZ_AP024269:2643905-2644309 [Kaistia sp. 32K]
ATGCGCTATGTCGCCTTCATCCATAAGGACCCCGGGAGCGTCTACGGCGTCAGCTTCCCGGATTTCCCCGGCTGCATCTC
GGCCGGCGATACCGCCGACGAGGCCGTCGAACAGGCCGCCGAGGCGCTCTCCGGCCATGTCGCCATGATGGCGGCGGATG
GCGATGCCATTCCGGCGCCCCGCTCGCTCGAGGAAATTCTCGCGGATCCCGCGATCGAGGAAGAGCGGGAGGGAGCGAGC
TTCGCCTTCGTGCCGCTGATCCGCGAACAGGGCGCCAAGGTGCCGGTGAACATCTCGCTCGACGCCGGCCTGCTCGAGTC
GATCGACGAGGCCGCTCGCAAGCGCGGCATCACCCGGTCGGCATTCCTGGCGAGCGCGGCCCGGAAGGAGATCGTAGCGG
CCTAG