Detailed information of TA system TA038271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2703846..2704510
• Replicon: chromosome
• Accession: NZ_AP024266
• Organism: Mycobacterium intracellulare strain M.i.27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IUS5
• Locus tag: JN183_RS12930
• Protein ID: WP_014379897.1
• Coordinates: 2704103..2704510 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN183_RS12925
• Protein ID: WP_009954496.1
• Coordinates: 2703846..2704106 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN183_RS12900	2700361..2700852	-	492	WP_095562636.1	DUF1707 domain-containing protein	-
JN183_RS12905	2700922..2701518	+	597	WP_095562637.1	TetR/AcrR family transcriptional regulator	-
JN183_RS12910	2701523..2702791	-	1269	WP_095562638.1	cytochrome P450	-
JN183_RS12915	2702951..2703403	+	453	WP_014379896.1	VOC family protein	-
JN183_RS12920	2703435..2703723	+	289	Protein_2548	hypothetical protein	-
JN183_RS12925	2703846..2704106	+	261	WP_009954496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JN183_RS12930	2704103..2704510	+	408	WP_014379897.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN183_RS12935	2704688..2707789	+	3102	WP_014379898.1	DEAD/DEAH box helicase	-
JN183_RS12940	2707850..2709415	-	1566	WP_014379899.1	serine/threonine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14307.31 Da
• Isoelectric Point: 4.2772

>T38271 WP_014379897.1 NZ_AP024266:2704103-2704510 [Mycobacterium intracellulare]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPIDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T38271 NZ_AP024266:2704103-2704510 [Mycobacterium intracellulare]
ATGAGCAACCCGCAGGTGGCCGGAGTGCTCGACACTTCGGTGTTCATCGCGACCGAAAGTGGTCGGCCTCTCGACGCGGC
GCTGATCCCTGATCAAGTTGGCACGACGGTTGTTACCCTCGCGGAACTGCACGTTGGTGTGTTGGCCGCGTCGACTTCCG
ATATTAGAGCGCAGCGCCTCGCGACATTGGAATCCATTGCGGATATCGAAGCGCTGCCGATCGATGACGATGCGGCCCGG
ATGTGGGCCCGGCTGCGGATCCATCTCGCCGAGACCGGTCAACGCGTCCGGATCAACGATCTGTGGATCGCGGCCATCGC
GGCGTCCCGAGGGCTACCCGTTGTCACGCAGGACGATGACTTTGCCGTCTTGGATGGTGCGGCCAATTTGACCGTTATCC
GCGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT38271 WP_009954496.1 NZ_AP024266:2703846-2704106 [Mycobacterium intracellulare]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT38271 NZ_AP024266:2703846-2704106 [Mycobacterium intracellulare]
ATGTCTGAAGTGGCATCTCGTGAGCTGCGCAACGATACGGCTGGGGTGCTGCGGCGGGTTCGGGATGGAGAGGACGTCAC
CATCACTGTCAGTGGCCGACCGGTCGCAGTTCTTACCGCAGTTGTCCCTCAGCGCAGGCGTTGGCTGAGCAAGACGGAGT
TGCTGTCGCGCTTGCCCCGCGTTCAAGCGGATCCGGGATTACGCGAGGACCTTCACGCACTCGCCGGCGACACTACCGAG
GAGCTGGGCCCGATCCGATGA

Structures

Toxin

• AlphaFold DB: A0A7R7MTD0