Detailed information of TA system TA038257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2420301..2420965
• Replicon: chromosome
• Accession: NZ_AP024262
• Organism: Mycobacterium intracellulare strain M025

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IUS5
• Locus tag: JN216_RS11450
• Protein ID: WP_014379897.1
• Coordinates: 2420558..2420965 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN216_RS11445
• Protein ID: WP_009954496.1
• Coordinates: 2420301..2420561 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN216_RS11420	2416816..2417307	-	492	WP_095562636.1	DUF1707 domain-containing protein	-
JN216_RS11425	2417377..2417973	+	597	WP_095562637.1	TetR/AcrR family transcriptional regulator	-
JN216_RS11430	2417978..2419246	-	1269	WP_095562638.1	cytochrome P450	-
JN216_RS11435	2419406..2419858	+	453	WP_014379896.1	VOC family protein	-
JN216_RS11440	2419890..2420178	+	289	Protein_2255	hypothetical protein	-
JN216_RS11445	2420301..2420561	+	261	WP_009954496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JN216_RS11450	2420558..2420965	+	408	WP_014379897.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN216_RS11455	2421143..2424244	+	3102	WP_014379898.1	DEAD/DEAH box helicase	-
JN216_RS11460	2424305..2425870	-	1566	WP_014379899.1	serine/threonine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14307.31 Da
• Isoelectric Point: 4.2772

>T38257 WP_014379897.1 NZ_AP024262:2420558-2420965 [Mycobacterium intracellulare]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPIDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T38257 NZ_AP024262:2420558-2420965 [Mycobacterium intracellulare]
ATGAGCAACCCGCAGGTGGCCGGAGTGCTCGACACTTCGGTGTTCATCGCGACCGAAAGTGGTCGGCCTCTCGACGCGGC
GCTGATCCCTGATCAAGTTGGCACGACGGTTGTTACCCTCGCGGAACTGCACGTTGGTGTGTTGGCCGCGTCGACTTCCG
ATATTAGAGCGCAGCGCCTCGCGACATTGGAATCCATTGCGGATATCGAAGCGCTGCCGATCGATGACGATGCGGCCCGG
ATGTGGGCCCGGCTGCGGATCCATCTCGCCGAGACCGGTCAACGCGTCCGGATCAACGATCTGTGGATCGCGGCCATCGC
GGCGTCCCGAGGGCTACCCGTTGTCACGCAGGACGATGACTTTGCCGTCTTGGATGGTGCGGCCAATTTGACCGTTATCC
GCGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT38257 WP_009954496.1 NZ_AP024262:2420301-2420561 [Mycobacterium intracellulare]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT38257 NZ_AP024262:2420301-2420561 [Mycobacterium intracellulare]
ATGTCTGAAGTGGCATCTCGTGAGCTGCGCAACGATACGGCTGGGGTGCTGCGGCGGGTTCGGGATGGAGAGGACGTCAC
CATCACTGTCAGTGGCCGACCGGTCGCAGTTCTTACCGCAGTTGTCCCTCAGCGCAGGCGTTGGCTGAGCAAGACGGAGT
TGCTGTCGCGCTTGCCCCGCGTTCAAGCGGATCCGGGATTACGCGAGGACCTTCACGCACTCGCCGGCGACACTACCGAG
GAGCTGGGCCCGATCCGATGA

Structures

Toxin

• AlphaFold DB: A0A7R7MTD0