Detailed information of TA system TA038250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1159853..1160506
• Replicon: chromosome
• Accession: NZ_AP024260
• Organism: Mycobacterium intracellulare strain M023

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN198_RS05775
• Protein ID: WP_014379252.1
• Coordinates: 1160045..1160506 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: JN198_RS05770
• Protein ID: WP_009957321.1
• Coordinates: 1159853..1160041 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN198_RS05760	1155826..1157403	+	1578	WP_014379250.1	serine hydrolase	-
JN198_RS05765	1157400..1159787	+	2388	WP_014379251.1	cation-translocating P-type ATPase	-
JN198_RS05770	1159853..1160041	+	189	WP_009957321.1	antitoxin	Antitoxin
JN198_RS05775	1160045..1160506	+	462	WP_014379252.1	SRPBCC family protein	Toxin
JN198_RS05780	1160590..1161360	+	771	WP_014379253.1	VOC family protein	-
JN198_RS05785	1161434..1161868	+	435	WP_008253914.1	hypothetical protein	-
JN198_RS05790	1161873..1162361	-	489	WP_036458243.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
JN198_RS05795	1162361..1163887	-	1527	WP_014379255.1	carotenoid oxygenase family protein	-
JN198_RS05800	1163889..1165109	-	1221	WP_014379256.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16609.18 Da
• Isoelectric Point: 8.5617

>T38250 WP_014379252.1 NZ_AP024260:1160045-1160506 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVAVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T38250 NZ_AP024260:1160045-1160506 [Mycobacterium intracellulare]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCGCTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCGCTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGGCGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT38250 WP_009957321.1 NZ_AP024260:1159853-1160041 [Mycobacterium intracellulare]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT38250 NZ_AP024260:1159853-1160041 [Mycobacterium intracellulare]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTCGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCCGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: X8A187